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Anti-TPP2 Antibody

Rabbit polyclonal antibody to TPP2

     
  •  - Anti-TPP2 Antibody AP61632
    Immunohistochemical analysis of TPP2 staining in mouse lung formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
    detail
  • IF - Anti-TPP2 Antibody AP61632
    Immunofluorescent analysis of TPP2 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IF/IC, IHC
Primary Accession P29144
Other Accession Q64514
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 138350 Da
Additional Information
Gene ID 7174
Other Names Tripeptidyl-peptidase 2; TPP-2; Tripeptidyl aminopeptidase; Tripeptidyl-peptidase II; TPP-II
Target/Specificity Recognizes endogenous levels of TPP2 protein.
Dilution WB~~1:1000
IF/IC~~N/A
IHC~~1:100~500
Format Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name TPP2
Function Cytosolic tripeptidyl-peptidase that releases N-terminal tripeptides from polypeptides and is a component of the proteolytic cascade acting downstream of the 26S proteasome in the ubiquitin- proteasome pathway (PubMed:25525876, PubMed:30533531). It plays an important role in intracellular amino acid homeostasis (PubMed:25525876). Stimulates adipogenesis (By similarity).
Cellular Location Cytoplasm. Nucleus. Note=Translocates to the nucleus in response to gamma-irradiation
Research Areas
Citations (0)
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Background

Recombinant full length protein of human TPP2

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$ 385.00
$ 195.00
Cat# AP61632
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