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CLC4 Antibody (C-term)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| IF, IHC-P-Leica, WB, E |
|---|---|
| Primary Accession | P51793 |
| Other Accession | P51794, Q61418 |
| Reactivity | Human |
| Predicted | Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 84917 Da |
| Antigen Region | 663-689 aa |
| Gene ID | 1183 |
|---|---|
| Other Names | H(+)/Cl(-) exchange transporter 4, Chloride channel protein 4, ClC-4, Chloride transporter ClC-4, CLCN4 |
| Target/Specificity | This CLC4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 663-689 amino acids from the C-terminal region of human CLC4. |
| Dilution | IF~~1:10~50 IHC-P-Leica~~1:10~50 WB~~1:1000 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | CLC4 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | CLCN4 |
|---|---|
| Function | Strongly outwardly rectifying, electrogenic H(+)/Cl(-)exchanger which mediates the exchange of chloride ions against protons (PubMed:18063579, PubMed:23647072, PubMed:25644381, PubMed:27550844, PubMed:28972156). The CLC channel family contains both chloride channels and proton-coupled anion transporters that exchange chloride or another anion for protons (PubMed:29845874). The presence of conserved gating glutamate residues is typical for family members that function as antiporters (PubMed:29845874). |
| Cellular Location | Early endosome membrane {ECO:0000250|UniProtKB:P51794}; Multi-pass membrane protein. Late endosome membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. Lysosome membrane; Multi-pass membrane protein. Recycling endosome membrane; Multi-pass membrane protein. Note=Localizes to late endosome membrane, lysosome membrane and recycling endosome membrane in the presence of CLCN3 |
| Tissue Location | Abundant in skeletal muscle and also detectable in brain and heart |
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Provided below are standard protocols that you may find useful for product applications.
Background
The CLCN family of voltage-dependent chloride channel genes comprises nine members (CLCN1-7, Ka and Kb) which demonstrate quite diverse functional characteristics while sharing significant sequence homology. Chloride channel 4 has an evolutionary conserved CpG island and is conserved in both mouse and hamster. This gene is mapped in close proximity to APXL (Apical protein Xenopus laevis-like) and OA1 (Ocular albinism type I), which are both located on the human X chromosome at band p22.3. The physiological role of chloride channel 4 remains unknown but may contribute to the pathogenesis of neuronal disorders.
References
Wang, T., et al., Gastroenterology 126(4):1157-1166 (2004).
Lamb, F.S., et al., J. Mol. Cell. Cardiol. 31(3):657-666 (1999).
Dinulos, M.B., et al., Genomics 35(1):244-247 (1996).
Schnur, R.E., et al., Hum. Genet. 95(5):594-595 (1995).
van Slegtenhorst, M.A., et al., Hum. Mol. Genet. 3(4):547-552 (1994).
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