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>   home   >   Products   >   Primary Antibodies   >   Collagen III Monoclonal Antibody(Q76)   

Collagen III Monoclonal Antibody(Q76)

     
  • WB - Collagen III Monoclonal Antibody(Q76) AP63370
    Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1,Collagen III Monoclonal Antibody(Q76) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    detail
  • IHC - Collagen III Monoclonal Antibody(Q76) AP63370
    Immunohistochemical analysis of paraffin-embedded Rat-heart tissue. 1,Collagen III Monoclonal Antibody(Q76) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    detail
  • IHC - Collagen III Monoclonal Antibody(Q76) AP63370
    Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1,Collagen III Monoclonal Antibody(Q76) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    detail
  • IF - Collagen III Monoclonal Antibody(Q76) AP63370
    Immunofluorescence analysis of Mouse-liver tissue. 1,Collagen III Monoclonal Antibody(Q76)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
    detail
  • WB - Collagen III Monoclonal Antibody(Q76) AP63370
    Western blot analysis of Hela, diluted at 1:1000.
    detail
  • IHC - Collagen III Monoclonal Antibody(Q76) AP63370
    Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1,Collagen III Monoclonal Antibody(Q76) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    detail
  • IHC - Collagen III Monoclonal Antibody(Q76) AP63370
    Immunohistochemical analysis of paraffin-embedded Rat-heart tissue. 1,Collagen III Monoclonal Antibody(Q76) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    detail
  • IHC - Collagen III Monoclonal Antibody(Q76) AP63370
    Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1,Collagen III Monoclonal Antibody(Q76) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    detail
  • IF - Collagen III Monoclonal Antibody(Q76) AP63370
    Immunofluorescence analysis of Mouse-liver tissue. 1,Collagen III Monoclonal Antibody(Q76)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
    detail
  • WB - Collagen III Monoclonal Antibody(Q76) AP63370
    Western blot analysis of Hela, diluted at 1:1000.
    detail
  • SPECIFICATION
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  • BACKGROUND
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF
Primary Accession P02461
Reactivity Human, Mouse, Rat
Host Mouse
Clonality Monoclonal
Calculated MW 138564 Da
Additional Information
Gene ID 1281
Other Names COL3A1; Collagen alpha-1(III) chain
Dilution WB~~WB: 1:1000 IF 1:200 IHC 1:50-300
IHC-P~~N/A
IF~~WB: 1:1000 IF 1:200 IHC 1:50-300
Format PBS, pH 7.4, containing 0.09% (W/V) sodium azide as Preservative and 50% Glycerol.
Storage Conditions-20℃
Protein Information
Name COL3A1
Function Collagen type III occurs in most soft connective tissues along with type I collagen. Involved in regulation of cortical development. Is the major ligand of ADGRG1 in the developing brain and binding to ADGRG1 inhibits neuronal migration and activates the RhoA pathway by coupling ADGRG1 to GNA13 and possibly GNA12.
Cellular Location Secreted, extracellular space, extracellular matrix {ECO:0000255|PROSITE-ProRule:PRU00793}
Citations (0)
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Background

Collagen type III occurs in most soft connective tissues along with type I collagen. Involved in regulation of cortical development. Is the major ligand of ADGRG1 in the developing brain and binding to ADGRG1 inhibits neuronal migration and activates the RhoA pathway by coupling ADGRG1 to GNA13 and possibly GNA12.

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$ 385.00
Cat# AP63370
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