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α-tubulin (Acetyl Lys40) Monoclonal Antibody(4A8)

     
  • WB - α-tubulin (Acetyl Lys40) Monoclonal Antibody(4A8) AP63647
    Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1,α-tubulin (Acetyl Lys40) Monoclonal Antibody(4A8) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    detail
  • IHC - α-tubulin (Acetyl Lys40) Monoclonal Antibody(4A8) AP63647
    Immunohistochemical analysis of paraffin-embedded Rat-heart tissue. 1,α-tubulin (Acetyl Lys40) Monoclonal Antibody(4A8) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    detail
  • IHC - α-tubulin (Acetyl Lys40) Monoclonal Antibody(4A8) AP63647
    Immunohistochemical analysis of paraffin-embedded Mouse-heart tissue. 1,α-tubulin (Acetyl Lys40) Monoclonal Antibody(4A8) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    detail
  • IF - α-tubulin (Acetyl Lys40) Monoclonal Antibody(4A8) AP63647
    Immunofluorescence analysis of Human-liver-cancer tissue. 1,α-tubulin (Acetyl Lys40) Monoclonal Antibody(4A8)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
    detail
  • IF - α-tubulin (Acetyl Lys40) Monoclonal Antibody(4A8) AP63647
    Immunofluorescence analysis of Mouse-lung tissue. 1,α-tubulin (Acetyl Lys40) Monoclonal Antibody(4A8)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
    detail
  • IF - α-tubulin (Acetyl Lys40) Monoclonal Antibody(4A8) AP63647
    Immunofluorescence analysis of Rat-spinal-cord tissue. 1,α-tubulin (Acetyl Lys40) Monoclonal Antibody(4A8)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
    detail
  • WB - α-tubulin (Acetyl Lys40) Monoclonal Antibody(4A8) AP63647
    Western blot analysis of extracts from Hela cells, untreated (-) or treated with TSA (1μM, 18 hr; +), using Acetyl- a-tubulin(Lys40) Mouse mAb 1:2000.
    detail
  • WB - α-tubulin (Acetyl Lys40) Monoclonal Antibody(4A8) AP63647
    Immunohistochemical analysis of paraffin-embedded Human Breast Carcinoma using a-tubulin(Acetyl Lys40) Mouse mAb diluted at 1:200.
    detail
  • WB - α-tubulin (Acetyl Lys40) Monoclonal Antibody(4A8) AP63647
    Immunohistochemical analysis of paraffin-embedded Mouse Brain Tissue using a-tubulin(Acetyl Lys40) Mouse mAb diluted at 1:200.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF
Primary Accession P68363
Reactivity Human, Rat, Mouse
Host Mouse
Clonality Monoclonal
Calculated MW 50152 Da
Additional Information
Gene ID 10376
Other Names Tubulin alpha-1B chain (Alpha-tubulin ubiquitous) (Tubulin K-alpha-1) (Tubulin alpha-ubiquitous chain)
Dilution WB~~WB 1:1000-2000, IHC 1:50-100 IF 1:200
IHC-P~~N/A
IF~~WB 1:1000-2000, IHC 1:50-100 IF 1:200
Format Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.09% (W/V) sodium azide.
Storage Conditions-20℃
Protein Information
Name TUBA1B
Function Tubulin is the major constituent of microtubules, protein filaments consisting of alpha- and beta-tubulin heterodimers (PubMed:38305685, PubMed:34996871, PubMed:38609661). Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms (PubMed:38305685, PubMed:34996871, PubMed:38609661). Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin (PubMed:34996871, PubMed:38609661).
Cellular Location Cytoplasm, cytoskeleton
Citations ( 0 )

Background

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

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$ 385.00
Cat# AP63647
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Availability: 4 weeks
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