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FLI1 Antibody (N-term K67)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - FLI1 Antibody (N-term K67) AP6514D
    Western blot analysis of FLI1 antibody (N-term) (Cat. #AP6514d) in 293 cell line lysates (35ug/lane). FLI1 (arrow) was detected using the purified Pab.
    detail
  • IHC-P - FLI1 Antibody (N-term K67) AP6514D
    FLI-1 Antibody (N-term) (Cat. #AP6514d) IHC analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the FLI-1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession Q01543
Other Accession P26323, Q29RS8
Reactivity Human
Predicted Bovine, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 50982 Da
Antigen Region 52-80 aa
Additional Information
Gene ID 2313
Other Names Friend leukemia integration 1 transcription factor, Proto-oncogene Fli-1, Transcription factor ERGB, FLI1
Target/Specificity This FLI1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 52-80 amino acids from the N-terminal region of human FLI1.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsFLI1 Antibody (N-term K67) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name FLI1
Function Sequence-specific transcriptional activator (PubMed:24100448, PubMed:26316623, PubMed:28255014). Recognizes the DNA sequence 5'- C[CA]GGAAGT-3'.
Cellular Location Nucleus.
Research Areas
Citations (0)
citation

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Background

FLI1 is a sequence-specific transcriptional activator. It recognizes the DNA sequence 5'-C[CA]GGAAGT-3'.

References

Embree,L.J., Cancer Res. 69 (10), 4363-4371 (2009)
Torlakovic,E.E., Histol. Histopathol. 23 (11), 1309-1314 (2008)
Cironi,L., PLoS ONE 3 (7), E2634 (2008)

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$ 182.50
$ 70.00
Cat# AP6514D
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