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HMGCR Antibody (Center)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 2
- PROTOCOLS
- BACKGROUND
Application 
| IHC-P, WB, E |
|---|---|
| Primary Accession | P04035 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 97476 Da |
| Antigen Region | 380-410 aa |
| Gene ID | 3156 |
|---|---|
| Other Names | 3-hydroxy-3-methylglutaryl-coenzyme A reductase, HMG-CoA reductase, HMGCR |
| Target/Specificity | This HMGCR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 380~410 amino acids from the Center region of human HMGCR. |
| Dilution | IHC-P~~1:50~100 WB~~1:1000 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | HMGCR Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | HMGCR (HGNC:5006) |
|---|---|
| Function | Catalyzes the conversion of (3S)-hydroxy-3-methylglutaryl-CoA (HMG-CoA) to mevalonic acid, the rate-limiting step in the synthesis of cholesterol and other isoprenoids, thus plays a critical role in cellular cholesterol homeostasis (PubMed:21357570, PubMed:2991281, PubMed:36745799, PubMed:6995544). HMGCR is the main target of statins, a class of cholesterol-lowering drugs (PubMed:11349148, PubMed:18540668, PubMed:36745799). |
| Cellular Location | Endoplasmic reticulum membrane; Multi-pass membrane protein {ECO:0000250|UniProtKB:P00347}. Peroxisome membrane; Multi-pass membrane protein {ECO:0000250|UniProtKB:P00347} |
| Tissue Location | [Isoform 1]: Ubiquitously expressed with the highest levels in the cerebellum, fetal brain, testis, skin and adrenal gland. [Isoform 3]: Low abundance except in skin, esophagus, and uterine cervix. |
Provided below are standard protocols that you may find useful for product applications.
Background
HMG-CoA reductase is the rate-limiting enzyme for cholesterol synthesis and is regulated via a negative feedback mechanism mediated by sterols and non-sterol metabolites derived from mevalonate, the product of the reaction catalyzed by reductase. Normally in mammalian cells this enzyme is suppressed by cholesterol derived from the internalization and degradation of low density lipoprotein (LDL) via the LDL receptor. Competitive inhibitors of the reductase induce the expression of LDL receptors in the liver, which in turn increases the catabolism of plasma LDL and lowers the plasma concentration of cholesterol, an important determinant of atherosclerosis.
References
Chen,Y.C., Lipids 44 (8), 733-743 (2009)
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