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COVID19
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HTATSF1 Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - HTATSF1 Antibody (N-term) AP6654a
    Western blot analysis of HTATSF1 Antibody (N-term) (Cat. #AP6654a) in Jurkat cell line lysates (35ug/lane). HTATSF1 (arrow) was detected using the purified Pab.
    detail
  • IHC-P - HTATSF1 Antibody (N-term) AP6654a
    Formalin-fixed and paraffin-embedded human brain tissue with HTATSF1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB, E
Primary Accession O43719
Other Accession Q8BGC0, Q5H919
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 85853 Da
Antigen Region 1-30 aa
Additional Information
Gene ID 27336
Other Names HIV Tat-specific factor 1, Tat-SF1, HTATSF1
Target/Specificity This HTATSF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human HTATSF1.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHTATSF1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HTATSF1 {ECO:0000303|PubMed:35597237, ECO:0000312|HGNC:HGNC:5276}
Function Component of the 17S U2 SnRNP complex of the spliceosome, a large ribonucleoprotein complex that removes introns from transcribed pre-mRNAs (PubMed:30567737, PubMed:32494006, PubMed:34822310). The 17S U2 SnRNP complex (1) directly participates in early spliceosome assembly and (2) mediates recognition of the intron branch site during pre-mRNA splicing by promoting the selection of the pre-mRNA branch- site adenosine, the nucleophile for the first step of splicing (PubMed:30567737, PubMed:32494006, PubMed:34822310). Within the 17S U2 SnRNP complex, HTATSF1 is required to stabilize the branchpoint- interacting stem loop (PubMed:34822310). HTATSF1 is displaced from the 17S U2 SnRNP complex before the stable addition of the 17S U2 SnRNP complex to the spliceosome, destabilizing the branchpoint-interacting stem loop and allowing to probe intron branch site sequences (PubMed:32494006, PubMed:34822310). Also acts as a regulator of transcriptional elongation, possibly by mediating the reciprocal stimulatory effect of splicing on transcriptional elongation (PubMed:10454543, PubMed:10913173, PubMed:11780068). Involved in double-strand break (DSB) repair via homologous recombination in S- phase by promoting the recruitment of TOPBP1 to DNA damage sites (PubMed:35597237). Mechanistically, HTATSF1 is (1) recruited to DNA damage sites in S-phase via interaction with poly-ADP-ribosylated RPA1 and (2) phosphorylated by CK2, promoting recruitment of TOPBP1, thereby facilitating RAD51 nucleofilaments formation and RPA displacement, followed by homologous recombination (PubMed:35597237).
Cellular Location Nucleus. Chromosome Note=Recruited to DNA damage sites during S-phase following interaction with poly-ADP-ribosylated RPA1.
Tissue Location Widely expressed..
Research Areas
Citations (0)
citation

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Background

HTATSF1 functions as a cofactor for the stimulation of transcriptional elongation by HIV-1 Tat, which binds to the HIV-1 promoter through Tat-TAR interaction. This protein may also serve as a dual-function factor to couple transcription and splicing and to facilitate their reciprocal activation.

References

Miller,H.B., PLoS ONE 4 (5), E5710 (2009)
Remoli,A.L., Biochem. J. 396 (2), 371-380 (2006)

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Anonymous
2016-08-23 21:15:32
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Species tested
Mouse,Rat
Application tested
WB
Brief protocol
1. Block with 3% skim milk for 1 hour at room temperature. 2. Incubate overnight with Abgent primary antibody 1:1000 in 3% skim milk at 4℃ 3. Wash 5*5 min with TBST. 4. Incubate with HRP-conjugated secondary antibody 1:5000 in 3% skim milk for 1 hour at room temperature. 5. Wash 5*5 min with TBST. 6. Incubate with ECL substrates and expose
$ 182.50
$ 70.00
Cat# AP6654a
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