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CHC1L Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - CHC1L Antibody (N-term) AP6741a
    Anti-CHC1L Antibody (N-term) at 1:2000 dilution + Molt-4 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 60 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IHC-P - CHC1L Antibody (N-term) AP6741a
    Formalin-fixed and paraffin-embedded human brain tissue reacted with CHC1L Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
  • FC - CHC1L Antibody (N-term) AP6741a
    CHC1L Antibody (N-term) (Cat. #AP6741a) flow cytometric analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, FC, IHC-P, E
Primary Accession O95199
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 60315 Da
Antigen Region 71-99 aa
Additional Information
Gene ID 1102
Other Names RCC1 and BTB domain-containing protein 2, Chromosome condensation 1-like, CHC1-L, RCC1-like G exchanging factor, Regulator of chromosome condensation and BTB domain-containing protein 2, RCBTB2, CHC1L, RLG
Target/Specificity This CHC1L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 71-99 amino acids from the N-terminal region of human CHC1L.
Dilution WB~~1:2000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCHC1L Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RCBTB2
Synonyms CHC1L, RLG
Cellular Location Cytoplasmic vesicle, secretory vesicle, acrosome {ECO:0000250|UniProtKB:Q99LJ7}. Note=Mainly found in the acrosomal cap region. {ECO:0000250|UniProtKB:Q99LJ7}
Research Areas
Citations (0)
citation

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Background

RCBTB2 is a member of the RCC1-related GEF family. The N-terminal half of the amino acid sequence shows similarity to the regulator of chromosome condensation RCC1, which acts as a guanine nucleotide exchange factor (GEF) protein for the Ras-related GTPase Ran.

References

Latil,A., Int. J. Cancer 99 (5), 689-696 (2002)

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$ 365.00
$ 140.00
Cat# AP6741a
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