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HOXB5 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - HOXB5 Antibody (C-term) AP6834b
    Western blot analysis of lysates from zebra fish muscle, Zebrafish tissue lysate (from left to right), using DANRE HOXD4 Antibody(Cat. #AP6834b). AP6834b was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
    detail
  • WB - HOXB5 Antibody (C-term) AP6834b
    HOXB5 Antibody (C-term) (Cat. #AP6834b) western blot analysis in A549,HUVEC cell line lysates (35ug/lane).This demonstrates the HOXB5 antibody detected the HOXB5 protein (arrow).
    detail
  • FC - HOXB5 Antibody (C-term) AP6834b
    Flow cytometric analysis of widr cells using HOXB5 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, FC, E
Primary Accession P09067
Other Accession P09077, P10628, P09016, P17278, O57374, P02832, P10629, P09630, P15862, P09020, P32043, Q00444, P09074, P04476, Q91771, P14839, Q9YGT4, P15861, P09019, P09079, P14838, P09013, P09014, P09071, P09634, P02830, P31268, Q90VZ9, P09092, P31267, Q5YLH5, P52949
Reactivity Human
Predicted Drosophila, Zebrafish, Chicken, Mouse, Sheep, Bovine, Rat, Xenopus
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 29434 Da
Antigen Region 193-222 aa
Additional Information
Gene ID 3215
Other Names Homeobox protein Hox-B5, Homeobox protein HHOC10, Homeobox protein Hox-2A, Homeobox protein Hu-1, HOXB5, HOX2A
Target/Specificity This HOXB5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 193-222 amino acids from the C-terminal region of human HOXB5.
Dilution WB~~1:1000
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHOXB5 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HOXB5
Synonyms HOX2A
Function Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
Cellular Location Nucleus.
Tissue Location Spinal cord.
Citations (0)
citation

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Background

HOXB5 is a nuclear protein with a homeobox DNA-binding domain. This protein functions as a sequence-specific transcription factor that is involved in lung and gut development.

References

Kosaki,K., et.al., Teratology 65 (2), 50-62 (2002)

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$ 182.50
$ 70.00
Cat# AP6834b
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