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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   GALT Antibody (C-term)   

GALT Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - GALT Antibody (C-term) AP6880b
    Anti-GALT Antibody (C-term) at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 43 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • WB - GALT Antibody (C-term) AP6880b
    Western blot analysis of GALT Antibody (C-term) (Cat. #AP6880b) in MDA-MB231 cell line lysates (35ug/lane). GALT (arrow) was detected using the purified Pab.
    detail
  • IHC-P - GALT Antibody (C-term) AP6880b
    Formalin-fixed and paraffin-embedded human breast carcinoma reacted with GALT Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
  • FC - GALT Antibody (C-term) AP6880b
    Flow cytometric analysis of MDA-MB468 cells using GALT Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, IHC-P, WB, E
Primary Accession P07902
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 43363 Da
Antigen Region 344-373 aa
Additional Information
Gene ID 2592
Other Names Galactose-1-phosphate uridylyltransferase, Gal-1-P uridylyltransferase, UDP-glucose--hexose-1-phosphate uridylyltransferase, GALT
Target/Specificity This GALT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 344-373 amino acids from the C-terminal region of human GALT.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGALT Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GALT
Function Plays an important role in galactose metabolism.
Research Areas
Citations (0)
citation

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Background

Galactose-1-phosphate uridyl transferase (GALT) catalyzes the second step of the Leloir pathway of galactose metabolism, namely the conversion of UDP-glucose + galactose-1-phosphate to glucose-1-phosphate + UDP-galactose. The absence of this enzyme results in classic galactosemia in humans and can be fatal in the newborn period if lactose is not removed from the diet.

References

Leslie,N.D., et.al., Genomics 14 (2), 474-480 (1992)

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$ 182.50
$ 70.00
Cat# AP6880b
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