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Aurora-C Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - Aurora-C Antibody (N-term) AP7000D
    Anti-Aurora-C Antibody (N-term) at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 36 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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  • WB - Aurora-C Antibody (N-term) AP7000D
    The anti-Aurora C Pab (Cat. #AP7000d) is used in Western blot to detect Aurora C in lysates of 293 cells expressing Flag tag (lane 1), Flag-tagged Aurora A (lane 2), Flag-tagged Aurora B (lane 3), Flag-tagged Aurora C (lane 4), and in A375 cell lysate. In the immunofluorescence experiment, staining of HeLa cells expressing GFP-Aurora C is performed at different cellular mitotic stages with the anti-Aurora C Pab as primary antibody (column A), GFP fluorescence (column B), DAPI nuclear staining (column C), and anti-Aurora C merged to DAPI staining (column D). Data with Flagged Aurora is kindly provided by Drs. K. Sasai and S. Sen from the University of Texas MD Anderson Cancer Center (Houston, TX).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q9UQB9
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 35591 Da
Antigen Region 3-38 aa
Additional Information
Gene ID 6795
Other Names Aurora kinase C, Aurora 3, Aurora/IPL1-related kinase 3, ARK-3, Aurora-related kinase 3, Aurora/IPL1/Eg2 protein 2, Serine/threonine-protein kinase 13, Serine/threonine-protein kinase aurora-C, AURKC, AIE2, AIK3, AIRK3, ARK3, STK13
Target/Specificity This Aurora-C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 3-38 amino acids from the N-terminal region of human Aurora-C.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsAurora-C Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name AURKC
Synonyms AIE2, AIK3, AIRK3, ARK3, STK13
Function Serine/threonine-protein kinase component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Also plays a role in meiosis and more particularly in spermatogenesis. Has redundant cellular functions with AURKB and can rescue an AURKB knockdown. Like AURKB, AURKC phosphorylates histone H3 at 'Ser-10' and 'Ser-28'. AURKC phosphorylates the CPC complex subunits BIRC5/survivin and INCENP leading to increased AURKC activity. Phosphorylates TACC1, another protein involved in cell division, at 'Ser-228'.
Cellular Location Nucleus. Chromosome. Chromosome, centromere. Cytoplasm, cytoskeleton, spindle. Note=Distributes in the condensed chromosomes during prophase to metaphase. After entering anaphase, there is a dissociation from separated chromosomes and a redistribution to midzone microtubules, and finally remains in the midbody during cytokinesis.
Tissue Location Isoform 1 and isoform 2 are expressed in testis. Elevated expression levels were seen only in a subset of cancer cell lines such as Hep-G2, Huh-7 and HeLa. Expression is maximum at M phase
Research Areas
Citations ( 0 )

Background

Chromosomal segregation during mitosis as well as meiosis is regulated by kinases and phosphatases. The Aurora kinases, members of the Ser/Thr protein kinase family, associate with microtubules during chromosome movement and segregation. Aurora kinase C may play a part in organizing microtubules in relation to the function of the centrosome/spindle pole during mitosis. This protein is localized to centrosome from anaphase to cytokinesis. Expression is limited to testis in normal cells. Elevated expression levels are seen only in a subset of cancer cells such as HepG2, HuH7 and HeLa cells. Aurora-C expression is maximum at M phase.

References

Kimura, M., et al., J. Biol. Chem. 274(11):7334-7340 (1999).
Tseng, T.C., et al., DNA Cell Biol. 17(10):823-833 (1998).
Bernard, M., et al., Genomics 53(3):406-409 (1998).

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$ 365.00
$ 140.00
Cat# AP7000D
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