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MYT1 Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | Q99640 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 54521 Da |
| Gene ID | 9088 |
|---|---|
| Other Names | Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase, Myt1 kinase, PKMYT1, MYT1 |
| Target/Specificity | This MYT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding T495 of human MYT1. |
| Dilution | WB~~1:1000 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | MYT1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | PKMYT1 |
|---|---|
| Synonyms | MYT1 |
| Function | Acts as a negative regulator of entry into mitosis (G2 to M transition) by phosphorylation of the CDK1 kinase specifically when CDK1 is complexed to cyclins (PubMed:9268380, PubMed:9001210, PubMed:10504341, PubMed:10373560). Mediates phosphorylation of CDK1 predominantly on 'Thr-14'. Also involved in Golgi fragmentation (PubMed:9268380, PubMed:9001210). May be involved in phosphorylation of CDK1 on 'Tyr-15' to a lesser degree, however tyrosine kinase activity is unclear and may be indirect (PubMed:9268380, PubMed:9001210). |
| Cellular Location | Endoplasmic reticulum membrane; Peripheral membrane protein. Golgi apparatus membrane; Peripheral membrane protein |
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Provided below are standard protocols that you may find useful for product applications.
Background
The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase preferentially phosphorylates and inactivates cell division cycle 2 protein (CDC2), and thus negatively regulates cell cycle G2/M transition. This kinase is associated with the membrane throughout the cell cycle. Its activity is highly regulated during the cell cycle. Protein kinases AKT1/PKB and PLK (Polo-like kinase) have been shown to phosphorylate and regulate the activity of this kinase. Alternatively spliced transcript variants encoding distinct isoforms have been reported. Transcript Variant: This variant (1) encodes the longer isoform (1).
References
Dai, X., et al., J. Invest. Dermatol. 122(6):1356-1364 (2004).
Nakajima, H., et al., J. Biol. Chem. 278(28):25277-25280 (2003).
Passer, B.J., et al., Proc. Natl. Acad. Sci. U.S.A. 100(5):2284-2289 (2003).
Okumura, E., et al., Nat. Cell Biol. 4(2):111-116 (2002).
Booher, R.N., et al., J. Biol. Chem. 272(35):22300-22306 (1997).
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