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ADRB2 Antibody (S364)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - ADRB2 Antibody (S364) AP7263E
    All lanes : Anti-ADRB2 Antibody (S364) at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: HUVEC whole cell lysate Lane 4: Human liver lysate Lane 5: NCI-H460 whole cell lysate Lane 6: PC-3 whole cell lysate Lane 7: A431 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 46 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IHC-P - ADRB2 Antibody (S364) AP7263E
    Immunohistochemical analysis of AP7263E on paraffin-embedded Human hepato carcinoma tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
    detail
  • FC - ADRB2 Antibody (S364) AP7263E
    Overlay histogram showing A431 cells stained with AP7263E(green line). The cells were fixed with 2% paraformaldehyde and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at Room temperature. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB, FC, E
Primary Accession P07550
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 46459 Da
Antigen Region 345-373 aa
Additional Information
Gene ID 154
Other Names Beta-2 adrenergic receptor, Beta-2 adrenoreceptor, Beta-2 adrenoceptor, ADRB2, ADRB2R, B2AR
Target/Specificity This ADRB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 345-373 amino acids from human ADRB2.
Dilution WB~~1:1000
IHC-P~~1:100
FC~~1:25
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsADRB2 Antibody (S364) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ADRB2
Synonyms ADRB2R, B2AR
Function Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. The beta-2-adrenergic receptor binds epinephrine with an approximately 30- fold greater affinity than it does norepinephrine.
Cellular Location Cell membrane; Multi-pass membrane protein. Early endosome. Golgi apparatus. Note=Colocalizes with VHL at the cell membrane (PubMed:19584355). Activated receptors are internalized into endosomes prior to their degradation in lysosomes (PubMed:20559325) Activated receptors are also detected within the Golgi apparatus (PubMed:27481942).
Research Areas
Citations (0)
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Background

ADRB2, beta-2-adrenergic receptor which is a member of the G protein-coupled receptor superfamily. This receptor is directly associated with one of its ultimate effectors, the class C L-type calcium channel Ca(V)1.2. This receptor-channel complex also contains a G protein, an adenylyl cyclase, cAMP-dependent kinase, and the counterbalancing phosphatase, PP2A. The assembly of the signaling complex provides a mechanism that ensures specific and rapid signaling by this G protein-coupled receptor. This protein is intronless.

References

Kobilka B.K., Dixon R.A.F.Proc. Natl. Acad. Sci. U.S.A. 84:46-50(1987)
Emorine L.J., Marullo S.Proc. Natl. Acad. Sci. U.S.A. 84:6995-6999(1987)
Kobilka B.K., Frielle T.J. Biol. Chem. 262:7321-7327(1987)

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$ 365.00
$ 140.00
Cat# AP7263E
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