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FARSA Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - FARSA Antibody (N-term) AP7566a
    Western blot analysis of anti-FARSA Pab (Cat.#AP7566a) in K562 cell line lysates (35ug/lane).FARSA (arrow) was detected using the purified Pab.
    detail
  • IHC-P - FARSA Antibody (N-term) AP7566a
    Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with FARSA antibody (N-term) (Cat.#AP7566a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB, E
Primary Accession Q9Y285
Other Accession Q505J8, Q8C0C7
Reactivity Human
Predicted Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 57564 Da
Antigen Region 54-83 aa
Additional Information
Gene ID 2193
Other Names Phenylalanine--tRNA ligase alpha subunit, CML33, Phenylalanyl-tRNA synthetase alpha subunit, PheRS, FARSA, FARS, FARSL, FARSLA
Target/Specificity This FARSA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 54-83 amino acids from the N-terminal region of human FARSA.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsFARSA Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name FARSA
Synonyms FARS, FARSL, FARSLA
Cellular Location Cytoplasm {ECO:0000250|UniProtKB:Q505J8}.
Citations (0)
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Background

Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. FARSA is similar to the catalytic subunit of prokaryotic and Saccharomyces cerevisiae phenylalanyl-tRNA synthetases (PheRS). This protein has been shown to be expressed in a tumor-selective and cell cycle stage- and differentiation-dependent manner, the first member of the tRNA synthetase gene family shown to exhibit this type of regulated expression.

References

Gevaert,K., Nat. Biotechnol. 21 (5), 566-569 (2003)
Moor,N.,Protein Expr. Purif. 24 (2), 260-267 (2002)
Sen,S., Proc. Natl. Acad. Sci. U.S.A. 94 (12), 6164-6169 (1997)

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$ 182.50
$ 70.00
Cat# AP7566a
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