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> home > Products > Primary Antibodies > Antibody Collections > Mitochondrion > EARS2 Antibody (C-term)
EARS2 Antibody (C-term)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 1
- PROTOCOLS
- BACKGROUND
Application 
| IHC-P, WB, E |
|---|---|
| Primary Accession | Q5JPH6 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 58689 Da |
| Antigen Region | 495-523 aa |
| Gene ID | 124454 |
|---|---|
| Other Names | Probable glutamate--tRNA ligase, mitochondrial, Glutamyl-tRNA synthetase, GluRS, EARS2, KIAA1970 |
| Target/Specificity | This EARS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 495-523 amino acids from the C-terminal region of human EARS2. |
| Dilution | IHC-P~~1:10~50 WB~~1:1000 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | EARS2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | EARS2 (HGNC:29419) |
|---|---|
| Function | Non-discriminating glutamyl-tRNA synthetase that catalyzes aminoacylation of both mitochondrial tRNA(Glu) and tRNA(Gln) and participates in RNA aminoacylation for mitochondrial protein translation (PubMed:19805282). Attachs glutamate to tRNA(Glu) or tRNA(Gln) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) or tRNA(Gln) (PubMed:19805282). In vitro, cytoplasmic tRNA(Gln) is slightly glutamylated, but with low activity (PubMed:19805282). |
| Cellular Location | Mitochondrion matrix |
Research Areas
Citations ( 0 )
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
Background
Glutamyl-tRNA synthetase (GluRS or EARS2) a class I aminoacyl-tRNA synthetase (aaRS), is primarily responsible for the glutamylation of tRNAGlu. It is part of the ?minimal set?of seventeen aaRSs found in every living organism and its presence is essential for the viability of cells.
References
Bonnefond,L.,Biochemistry 44 (12), 4805-4816 (2005)
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$ 150.00
$ 385.00
Cat# AP7573b
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