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Tyrosyl tRNA synthetase (YARS) Antibody (C-term)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 1
- PROTOCOLS
- BACKGROUND
Application 
| IHC-P, WB, E |
|---|---|
| Primary Accession | P54577 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 59143 Da |
| Antigen Region | 450-479 aa |
| Gene ID | 8565 |
|---|---|
| Other Names | Tyrosine--tRNA ligase, cytoplasmic, Tyrosyl-tRNA synthetase, TyrRS, Tyrosine--tRNA ligase, cytoplasmic, N-terminally processed, YARS |
| Target/Specificity | This Tyrosyl tRNA synthetase (YARS) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 450-479 amino acids from the C-terminal region of human Tyrosyl tRNA synthetase (YARS). |
| Dilution | IHC-P~~1:10~50 WB~~1:1000 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | Tyrosyl tRNA synthetase (YARS) Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | YARS1 (HGNC:12840) |
|---|---|
| Function | Tyrosine--tRNA ligase that catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr) (Probable) (PubMed:25533949). Also acts as a positive regulator of poly-ADP-ribosylation in the nucleus, independently of its tyrosine--tRNA ligase activity (PubMed:25533949). Activity is switched upon resveratrol-binding: resveratrol strongly inhibits the tyrosine-- tRNA ligase activity and promotes relocalization to the nucleus, where YARS1 specifically stimulates the poly-ADP-ribosyltransferase activity of PARP1 (PubMed:25533949). |
| Cellular Location | Cytoplasm. Nucleus Note=Cytoplasmic in normal conditions (PubMed:25533949). Resveratrol- binding in response to serum starvation promotes relocalization to the nucleus (PubMed:25533949). |
Provided below are standard protocols that you may find useful for product applications.
Background
Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Tyrosyl-tRNA synthetase belongs to the class I tRNA synthetase family. Cytokine activities have also been observed for the human tyrosyl-tRNA synthetase, after it is split into two parts, an N-terminal fragment that harbors the catalytic site and a C-terminal fragment found only in the mammalian enzyme. The N-terminal fragment is an interleukin-8-like cytokine, whereas the released C-terminal fragment is an EMAP II-like cytokine.
References
Yang,X.L., Chem. Biol. 14 (12), 1323-1333 (2007)
Jordanova,A., Nat. Genet. 38 (2), 197-202 (2006)
Bonnefond,L., Biochemistry 44 (12), 4805-4816 (2005)
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