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A-RAF Antibody (ARAF1) (Center)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| IHC-P, WB, E |
|---|---|
| Primary Accession | P10398 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 67585 Da |
| Antigen Region | 256-286 aa |
| Gene ID | 369 |
|---|---|
| Other Names | Serine/threonine-protein kinase A-Raf, Proto-oncogene A-Raf, Proto-oncogene A-Raf-1, Proto-oncogene Pks, ARAF, ARAF1, PKS, PKS2 |
| Target/Specificity | This A-RAF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 256-286 amino acids from the Central region of human A-RAF. |
| Dilution | WB~~1:1000 IHC-P~~1:50~100 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | A-RAF Antibody (ARAF1) (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | ARAF |
|---|---|
| Synonyms | ARAF1, PKS, PKS2 |
| Function | Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May also regulate the TOR signaling cascade. Phosphorylates PFKFB2 (PubMed:36402789). |
| Tissue Location | Predominantly in urogenital tissues. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.
References
Wu, X., et al., J. Biol. Chem. 271(6):3265-3271 (1996).
Lee, J.E., et al., Genomics 20(1):43-55 (1994).
Popescu, N.C., et al., Oncogene 4(4):517-519 (1989).
Beck, T.W., et al., Nucleic Acids Res. 15(2):595-609 (1987).
Yin, X.L., et al., Mol. Cell. Biochem. 231 (1-2), 69-74 (2002).
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