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CDC25C Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - CDC25C Antibody (C-term) AP7862b
    Western blot analysis of anti-CDC25C Antibody (C-term) (Cat.#AP7862b) in K562 cell line lysates (35ug/lane).CDC25C(arrow) was detected using the purified Pab.
    detail
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P30307
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 53365 Da
Antigen Region 376-402 aa
Additional Information
Gene ID 995
Other Names M-phase inducer phosphatase 3, Dual specificity phosphatase Cdc25C, CDC25C
Target/Specificity This CDC25C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 376-402 amino acids from the C-terminal region of human CDC25C.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCDC25C Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CDC25C
Function Functions as a dosage-dependent inducer in mitotic control. Tyrosine protein phosphatase required for progression of the cell cycle. When phosphorylated, highly effective in activating G2 cells into prophase. Directly dephosphorylates CDK1 and activates its kinase activity.
Cellular Location Nucleus
Research Areas
Citations (0)
citation

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Background

CDC25C is a tyrosine phosphatase and belongs to the Cdc25 phosphatase family. It directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It is also thought to suppress p53-induced growth arrest.

References

Varmeh,S., Mol. Cancer Ther. 7 (12), 3789-3799 (2008)
Long,J.M., Cell Cycle 7 (19), 3062-3073 (2008)
Bonnet,J., Cell Cycle 7 (13), 1991-1998 (2008)

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$ 182.50
$ 70.00
Cat# AP7862b
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