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>   home   >   Products   >   Primary Antibodies   >   Antibody Collections   >   Catalog Updated   >   ALOX15 Antibody (C-term)   

ALOX15 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • IF - ALOX15 Antibody (C-term) AP7896B
    Confocal immunofluorescent analysis of ALOX15 Antibody (C-term)(Cat#AP7896b) with hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
  • WB - ALOX15 Antibody (C-term) AP7896B
    All lanes : Anti-ALOX15 Antibody (C-term) at 1:2000 dilution Lane 1: K562 whole cell lysate Lane 2: MDA-MB-453 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 75 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - ALOX15 Antibody (C-term) AP7896B
    ALOX15 Antibody (C-term) (Cat.#AP7896b) western blot analysis in Hela(lane 1),A549(lane 2),K562(lane 3),MDA-MB435(lane 4) cell line lysates (35ug/lane).This demonstrates the ALOX15 antibody detected the ALOX15 protein (arrow).
  • IHC-P - ALOX15 Antibody (C-term) AP7896B
    Formalin-fixed and paraffin-embedded human lymph tissue reacted with ALOX15 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IF, IHC-P, WB, E
Primary Accession P16050
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 74804 Da
Antigen Region 610-643 aa
Additional Information
Gene ID 246
Other Names Arachidonate 15-lipoxygenase, 15-LOX, 15-LOX-1, 12/15-lipoxygenase, Arachidonate 12-lipoxygenase, leukocyte-type, 12-LOX, Arachidonate omega-6 lipoxygenase, ALOX15, LOG15
Target/Specificity This ALOX15 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 610-643 amino acids from the C-terminal region of human ALOX15.
Dilution IF~~1:10~50
WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsALOX15 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ALOX15 (HGNC:433)
Synonyms LOG15
Function Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids generating a spectrum of bioactive lipid mediators (PubMed:23242647, PubMed:25293588, PubMed:1944593, PubMed:17052953, PubMed:8334154, PubMed:24282679). Converts arachidonic acid into 12- hydroperoxyeicosatetraenoic acid/12-HPETE and 15- hydroperoxyeicosatetraenoic acid/15-HPETE (PubMed:1944593, PubMed:17052953, PubMed:8334154, PubMed:24282679). Also converts linoleic acid into 13-hydroperoxyoctadecadienoic acid (PubMed:8334154). Converts epoxy fatty acids to hydroperoxy-epoxides derivatives followed by an intramolecular nucleophilic substitution between hydroperoxy and epoxy moieties leading to the formation of monocyclic endoperoxides (PubMed:25293588). May also act on (12S)-hydroperoxyeicosatetraenoic acid/(12S)-HPETE to produce hepoxilin A3 (By similarity). Probably plays an important role in the immune and inflammatory responses. Through the oxygenation of membrane-bound phosphatidylethanolamine in macrophages may favor clearance of apoptotic cells during inflammation by resident macrophages and prevent an autoimmune response associated with the clearance of apoptotic cells by inflammatory monocytes. In parallel, may regulate actin polymerization which is crucial for several biological processes, including macrophage function. May also regulate macrophage function through regulation of the peroxisome proliferator activated receptor signaling pathway (By similarity). Finally, it is also involved in the cellular response to IL13/interleukin-13 (PubMed:21831839). In addition to its role in the immune and inflammatory responses, may play a role in epithelial wound healing in the cornea maybe through production of lipoxin A4. May also play a role in endoplasmic reticulum stress response and the regulation of bone mass (By similarity).
Cellular Location Cytoplasm, cytosol. Cell membrane; Peripheral membrane protein. Lipid droplet. Note=Predominantly cytosolic; becomes enriched at membranes upon calcium binding (By similarity) Translocates from the cytosol to the plasma membrane when stimulated by IL13/interleukin-13 and in macrophages binding apoptotic cells (By similarity). {ECO:0000250|UniProtKB:P39654}
Tissue Location Detected in monocytes and eosinophils (at protein level). Expressed in airway epithelial cells
Research Areas
Citations (0)

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Background

ALOX15 converts arachidonic acid to 15S-hydroperoxyeicosatetraenoic acid. The protein also acts on C-12 of arachidonate as well as on linoleic acid.

References

Tang,Y., Int. J. Cancer 124 (7), 1545-1551 (2009)
Bevan,S., Stroke 40 (3), 696-701 (2009)
Zuo,X., J. Biol. Chem. 283 (46), 31341-31347 (2008)

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$ 325.00
$ 109.00
Cat# AP7896B
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