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MEKK4 Antibody (C-term)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 2
- PROTOCOLS
- BACKGROUND
Application 
| E |
|---|---|
| Primary Accession | Q9Y6R4 |
| Other Accession | O08648 |
| Reactivity | Human |
| Predicted | Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 181685 Da |
| Antigen Region | 1074-1104 aa |
| Gene ID | 4216 |
|---|---|
| Other Names | Mitogen-activated protein kinase kinase kinase 4, MAP three kinase 1, MAPK/ERK kinase kinase 4, MEK kinase 4, MEKK 4, MAP3K4, KIAA0213, MAPKKK4, MEKK4, MTK1 |
| Target/Specificity | This MEKK4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1074~1104 amino acids from the C-terminal region of human MEKK4. |
| Dilution | E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | MEKK4 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | MAP3K4 |
|---|---|
| Synonyms | KIAA0213, MAPKKK4, MEKK4, MTK1 |
| Function | Component of a protein kinase signal transduction cascade. Activates the CSBP2, P38 and JNK MAPK pathways, but not the ERK pathway. Specifically phosphorylates and activates MAP2K4 and MAP2K6. |
| Cellular Location | Cytoplasm, perinuclear region. Note=Localized in perinuclear vesicular-like structures, probably Golgi-associated vesicles. |
| Tissue Location | Expressed at high levels in heart, placenta, skeletal muscle and pancreas, and at lower levels in other tissues |
Provided below are standard protocols that you may find useful for product applications.
Background
The central core of each mitogen-activated protein kinase (MAPK) pathway is a conserved cascade of 3 protein kinases: an activated MAPK kinase kinase (MAPKKK) phosphorylates and activates a specific MAPK kinase (MAPKK), which then activates a specific MAPK. While the ERK MAPKs are activated by mitogenic stimulation, the CSBP2 and JNK MAPKs are activated by environmental stresses such as osmotic shock, UV irradiation, wound stress, and inflammatory factors. The MEKK4 protein, also called MTK1, contains a protein kinase catalytic domain at the C terminus. The N-terminal nonkinase domain may contain a regulatory domain. Expression of MEKK4 in mammalian cells activated the CSBP2 and JNK MAPK pathways, but not the ERK pathway. In vitro kinase studies indicated that recombinant MEKK4 can specifically phosphorylate and activate PRKMK6 and SERK1, MAPKKs that activate CSBP2 and JNK, respectively; it could not phosphorylate PRKMK1, an MAPKK that activates ERKs. MEKK4 is a major mediator of environmental stresses that activate the CSBP2 MAPK pathway, and a minor mediator of the JNK pathway. Northern blot analysis detected an approximately 6-kb transcript in various human tissues. RT-PCR identified a shorter form of MEKK4 mRNA that lacks 49 codons and is probably generated by alternative splicing.
References
Luo, W., et al., J. Biol. Chem. 278(39):37451-37458 (2003).
Takekawa, M., et al., EMBO J. 16(16):4973-4982 (1997).
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