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MEK3 (MAP2K3) Antibody (N-term)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 1
- PROTOCOLS
- BACKGROUND
Application 
| IHC-P, WB, E |
|---|---|
| Primary Accession | P46734 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 39318 Da |
| Antigen Region | 15-45 aa |
| Gene ID | 5606 |
|---|---|
| Other Names | Dual specificity mitogen-activated protein kinase kinase 3, MAP kinase kinase 3, MAPKK 3, MAPK/ERK kinase 3, MEK 3, Stress-activated protein kinase kinase 2, SAPK kinase 2, SAPKK-2, SAPKK2, MAP2K3, MEK3, MKK3, PRKMK3, SKK2 |
| Target/Specificity | This MEK3 (MAP2K3) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 15-45 amino acids from the N-terminal region of human MEK3 (MAP2K3). |
| Dilution | WB~~1:1000 IHC-P~~1:50~100 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | MEK3 (MAP2K3) Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | MAP2K3 |
|---|---|
| Synonyms | MEK3, MKK3, PRKMK3, SKK2 |
| Function | Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38. Part of a signaling cascade that begins with the activation of the adrenergic receptor ADRA1B and leads to the activation of MAPK14. |
| Tissue Location | Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues |
Provided below are standard protocols that you may find useful for product applications.
Background
MAP2K3 is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis.
References
Yustein, J.T., et al., Oncogene 22(40):6129-6141 (2003).
Edlund, S., et al., Mol. Biol. Cell 14(2):529-544 (2003).
Lim, S., et al., J. Biol. Chem. 277(28):25040-25046 (2002).
Han, Q., et al., J. Biol. Chem. 277(50):48379-48385 (2002).
Wang, W., et al., Mol. Cell. Biol. 22(10):3389-3403 (2002).
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