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>   home   >   Products   >   Primary Antibodies   >   Cancer   >   Recombinant PD-L1 antibody [AC37]   

Recombinant PD-L1 antibody [AC37]

Rabbit Monoclonal Antibody (Mab)

     
  • WB - Recombinant PD-L1 antibody [AC37] AP80075
    All lanes : Anti-PDL-1 Antibody at 1:1000 dilution Lane 1: HEK 293 transfected with PDL1 whole cell lysate Lane 2: 293 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary: Goat Anti-Rabbit IgG (H&L) HRP. Predicted band size : 33 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IHC-P - Recombinant PD-L1 antibody [AC37] AP80075
    Immunohistochemical analysis of paraffin-embedded human tonsil tissue using AP80075.Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody for 15 min at room temperature. Secondary: Goat Anti-Rabbit IgG (H&L)
    detail
  • IHC-P - Recombinant PD-L1 antibody [AC37] AP80075
    Immunohistochemical analysis of paraffin-embedded human tonsil tissue using AP80075. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody for 15 min at room temperature. Secondary: Goat Anti-Rabbit IgG (H&L)
    detail
  • IHC-P - Recombinant PD-L1 antibody [AC37] AP80075
    Immunohistochemical analysis of paraffin-embedded human esophageal squamous carcinoma tissue using AP80075. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody for 15 min at room temperature. Secondary: Goat Anti-Rabbit IgG (H&L)
    detail
  • IHC-P - Recombinant PD-L1 antibody [AC37] AP80075
    Immunohistochemical analysis of paraffin-embedded NCI-H226 (left) and HEK 293 transfected with PD-L1(right) using AP80075. Cell was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody for 15 min at room temperature. Secondary: Goat Anti-Rabbit IgG (H&L)
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession Q9NZQ7
Reactivity Human
Host Rabbit
Clonality Monoclonal
Isotype IgG
Calculated MW 33275 Da
Additional Information
Gene ID 29126
Other Names Programmed cell death 1 ligand 1, PD-L1, PDCD1 ligand 1, Programmed death ligand 1, B7 homolog 1, B7-H1, CD274, CD274, B7H1, PDCD1L1, PDCD1LG1, PDL1, PDL-1
Target/Specificity Recombinant anti-PD-L1 monoclonal antibody recognizes endogenous levels of total PD-L1 protein.
Dilution WB~~1:1000
IHC-P~~1:100-1:400
Format Purified recombination monoclonal antibody supplied in PBS with 0.05% (W/V) sodium azide, and 0.05% BSA. This antibody is purified through a protein A column.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRecombinant PD-L1 antibody [AC37] is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CD274 (HGNC:17635)
Function Plays a critical role in induction and maintenance of immune tolerance to self (PubMed:11015443, PubMed:28813417, PubMed:28813410). As a ligand for the inhibitory receptor PDCD1/PD-1, modulates the activation threshold of T-cells and limits T-cell effector response (PubMed:11015443, PubMed:28813417, PubMed:28813410). Through a yet unknown activating receptor, may costimulate T-cell subsets that predominantly produce interleukin-10 (IL10) (PubMed:10581077).
Cellular Location Cell membrane; Single-pass type I membrane protein. Early endosome membrane; Single-pass type I membrane protein. Recycling endosome membrane; Single-pass type I membrane protein. Note=Associates with CMTM6 at recycling endosomes, where it is protected from being targeted for lysosomal degradation. [Isoform 2]: Endomembrane system; Single-pass type I membrane protein
Tissue Location Highly expressed in the heart, skeletal muscle, placenta and lung. Weakly expressed in the thymus, spleen, kidney and liver. Expressed on activated T- and B-cells, dendritic cells, keratinocytes and monocytes.
Research Areas
Citations (0)
citation

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Background

Programmed cell death 1 ligand 1 (PD-L1, B7-H1, CD274) is a member of the B7 family of cell surface ligands that regulate T cell activation and immune responses. The PD-L1 ligand binds the PD-1 transmembrane receptor and inhibits T cell activation. PD-L1 was discovered following a search for novel B7 protein homologs and was later shown to be expressed by antigen presenting cells, activated T cells, and tissues including placenta, heart, and lung. Similar in structure to related B7 family members, PD-L1 protein contains extracellular IgV and IgC domains and a short, cytoplasmic region. Research studies demonstrate that PD-L1 is expressed in several tumor types, including melanoma, ovary, colon, lung, breast, and renal cell carcinomas. Expression of PD-L1 in cancer is associated with tumor-infiltrating lymphocytes, which mediate PD-L1 expression through the release of interferon gamma. Additional research links PD-L1 expression to cancers associated with viral infections. Involved in the costimulatory signal, essential for T- cell proliferation and production of IL10 and IFNG, in an IL2- dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production.

References

Dong H.,et al.Nat. Med. 5:1365-1369(1999).
Freeman G.J.,et al.J. Exp. Med. 192:1027-1034(2000).
He X.-H.,et al.Acta Pharmacol. Sin. 26:462-468(2005).
Chi X.-Y.,et al.Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Dong, H. et al. (1999) Nat Med 5, 1365-9.
Freeman, G.J. et al. (2000) J Exp Med 192, 1027-34.
Liang, S.C. et al. (2003) Eur J Immunol 33, 2706-16.
Dong, H. et al. (2002) Nat Med 8, 793-800.
Thompson, R.H. et al. (2006) Cancer Res 66, 3381-5.
Pardoll, D.M. (2012) Nat Rev Cancer 12, 252-64.
Taube, J.M. et al. (2012) Sci Transl Med 4, 127ra37.
Lyford-Pike, S. et al. (2013) Cancer Res 73, 1733-41.
Chen, B.J. et al. (2013) Clin Cancer Res 19, 3462-73.
Wimberly, H. et al. (2014) Cancer Immunol Res , .

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$ 380.00
Cat# AP80075
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