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GNA12 Antibody (S67)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | Q03113 |
| Other Accession | P25157, Q6Q7Y5, P27601, Q14344, Q63210, P27600, Q5YKG4 |
| Reactivity | Human |
| Predicted | Zebrafish, Mouse, Rat, Drosophila |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 44279 Da |
| Antigen Region | 46-75 aa |
| Gene ID | 2768 |
|---|---|
| Other Names | Guanine nucleotide-binding protein subunit alpha-12, G alpha-12, G-protein subunit alpha-12, GNA12 |
| Target/Specificity | This GNA12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 46-75 amino acids from human GNA12. |
| Dilution | WB~~1:1000 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | GNA12 Antibody (S67) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | GNA12 |
|---|---|
| Function | Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems (PubMed:22609986, PubMed:15525651, PubMed:15240885, PubMed:17565996, PubMed:12515866, PubMed:16787920, PubMed:16705036, PubMed:23762476, PubMed:27084452). Activates effector molecule RhoA by binding and activating RhoGEFs (ARHGEF12/LARG) (PubMed:15240885, PubMed:12515866, PubMed:16202387). GNA12-dependent Rho signaling subsequently regulates transcription factor AP-1 (activating protein-1) (By similarity). GNA12-dependent Rho signaling also regulates protein phosphatese 2A activation causing dephosphorylation of its target proteins (PubMed:15525651, PubMed:17565996). Promotes tumor cell invasion and metastasis by activating RhoA/ROCK signaling pathway and up-regulating pro-inflammatory cytokine production (PubMed:23762476, PubMed:16787920, PubMed:16705036, PubMed:27084452). Inhibits CDH1-mediated cell adhesion in process independent from Rho activation (PubMed:11976333, PubMed:16787920). Together with NAPA promotes CDH5 localization to plasma membrane (PubMed:15980433). May play a role in the control of cell migration through the TOR signaling cascade (PubMed:22609986). |
| Cellular Location | Cell membrane; Lipid-anchor {ECO:0000250|UniProtKB:Q63210}. Lateral cell membrane; Lipid-anchor {ECO:0000250|UniProtKB:Q63210}. Cytoplasm. Note=CDH1 enhances cell membrane localization. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
Guanine nucleotide-binding proteins (G proteins) are modulators or transducers in various transmembrane signaling systems. G proteins are composed of 3 subunits; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site.
References
Krakstad, B.F., et al., Proc. Natl. Acad. Sci. U.S.A. 101(28):10314-10319 (2004).
Hillier, L.W., et al., Nature 424(6945):157-164 (2003).
Scherer, S.W., et al., Science 300(5620):767-772 (2003).
Suzuki, N., et al., Proc. Natl. Acad. Sci. U.S.A. 100(2):733-738 (2003).
Yamaguchi, Y., et al., J. Biol. Chem. 278(17):14936-14939 (2003).
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