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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   STEP / PTPN5 Antibody (N-Term)   

STEP / PTPN5 Antibody (N-Term)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - STEP / PTPN5 Antibody (N-Term) AP8430a
    Western blot analysis of hSTEP-Q155 (Cat. #AP8430a) in mouse brain tissue lysates (35ug/lane). STEP (arrow) was detected using the purified Pab
  • WB - STEP / PTPN5 Antibody (N-Term) AP8430a
    Western blot analysis of hSTEP-Q155 (Cat. #AP8430a) in HepG2 cell line lysates (35ug/lane). STEP (arrow) was detected using the purified Pab.
  • IHC-P - STEP / PTPN5 Antibody (N-Term) AP8430a
    Formalin-fixed and paraffin-embedded human brain tissue reacted with STEP antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P54829
Other Accession P54830
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 63538 Da
Antigen Region 168-198 aa
Additional Information
Gene ID 84867
Other Names Tyrosine-protein phosphatase non-receptor type 5, Neural-specific protein-tyrosine phosphatase, Striatum-enriched protein-tyrosine phosphatase, STEP, PTPN5
Target/Specificity This STEP / PTPN5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 168-198 amino acids from the N-terminal region of human STEP / PTPN5.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSTEP / PTPN5 Antibody (N-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PTPN5
Function May regulate the activity of several effector molecules involved in synaptic plasticity and neuronal cell survival, including MAPKs, Src family kinases and NMDA receptors.
Cellular Location Endoplasmic reticulum membrane; Multi-pass membrane protein
Research Areas
Citations (0)

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STEP (striatum-enriched phosphatase) is a neural-specific protein-tyrosine phosphatase, first isolated from the rat brain. The 537-amino acid predicted human protein as isolated from cDNA sequences is between 85 and 90% identical to the mouse and rat sequences. In rat neuronal cell cultures, glutamate-mediated activation of N-methyl-D-aspartate (NMDA) receptors leads to the rapid but transient phosphorylation of extracellular signal-related kinase-2 (ERK2). NMDA-mediated influx of calcium, activates the calcium-dependent phosphatase calcineurin and the resulting dephosphorylation and activation of STEP. STEP then inactivatea ERK2 through tyrosine dephosphorylation and blocks translocation of the kinase to the nucleus. STEP plays a significant role in regulating the ERK activation and downstream signaling in neurons.


Ota, T., et al., Nat. Genet. 36(1):40-45 (2004).
Li, X., et al., Genomics 28(3):442-449 (1995).

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$ 325.00
$ 109.00
Cat# AP8430a
Availability: In Stock
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