- CITATIONS: 1
|Application ||FC, IHC-P, WB, E|
|Other Accession||P41499, P35235, Q90687, NP_002825|
|Predicted||Chicken, Mouse, Rat|
|Calculated MW||68011 Da|
|Antigen Region||526-551 aa|
|Other Names||Tyrosine-protein phosphatase non-receptor type 11, Protein-tyrosine phosphatase 1D, PTP-1D, Protein-tyrosine phosphatase 2C, PTP-2C, SH-PTP2, SHP-2, Shp2, SH-PTP3, PTPN11, PTP2C, SHPTP2|
|Target/Specificity||This SHP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 526-551 amino acids from human SHP2.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||SHP2 Antibody (Y546) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus (PubMed:10655584, PubMed:18559669, PubMed:18829466, PubMed:26742426, PubMed:28074573). Positively regulates MAPK signal transduction pathway (PubMed:28074573). Dephosphorylates GAB1, ARHGAP35 and EGFR (PubMed:28074573). Dephosphorylates ROCK2 at 'Tyr-722' resulting in stimulation of its RhoA binding activity (PubMed:18559669). Dephosphorylates CDC73 (PubMed:26742426). Dephosphorylates SOX9 on tyrosine residues, leading to inactivate SOX9 and promote ossification (By similarity).|
|Cellular Location||Cytoplasm. Nucleus|
|Tissue Location||Widely expressed, with highest levels in heart, brain, and skeletal muscle.|
Provided below are standard protocols that you may find useful for product applications.
SHP2, also known as PTPN11, is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains two tandem Src homology-2 domains, which function as phospho-tyrosine binding domains and mediate the interaction of this PTP with its substrates. This PTP is widely expressed in most tissues and plays a regulatory role in various cell signaling events that are important for a diversity of cell functions, such as mitogenic activation, metabolic control, transcription regulation, and cell migration. Mutations in the gene are a cause of Noonan syndrome as well as acute myeloid leukemia.
Chan, R.J., et al., Blood 105(9):3737-3742 (2005).
Sturla, L.M., et al., J. Biol. Chem. 280(15):14597-14604 (2005).
Loh, M.L., et al., Leuk. Res. 29(4):459-462 (2005).
Wang, Q., et al., J. Biol. Chem. 280(9):8397-8406 (2005).
Niihori, T., et al., J. Hum. Genet. 50(4):192-202 (2005).
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