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>   home   >   Products   >   Primary Antibodies   >   Antibody Collections   >   Interferon-gamma signaling   >   SHP2 Antibody (Y546)   

SHP2 Antibody (Y546)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - SHP2 Antibody (Y546) AP8471e
    Western blot analysis of SHP2 Antibody (Y546) (Cat. #AP8471e) in 293, K562 cell line lysates (35ug/lane). SHP2 (arrow) was detected using the purified Pab.
  • IHC-P - SHP2 Antibody (Y546) AP8471e
    Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with SHP2 Antibody (Y546), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - SHP2 Antibody (Y546) AP8471e
    Flow cytometric analysis of 293 cells using SHP2 Antibody (Y546)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, IHC-P, WB, E
Primary Accession Q06124
Other Accession P41499, P35235, Q90687, NP_002825
Reactivity Human
Predicted Chicken, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 68011 Da
Antigen Region 526-551 aa
Additional Information
Gene ID 5781
Other Names Tyrosine-protein phosphatase non-receptor type 11, Protein-tyrosine phosphatase 1D, PTP-1D, Protein-tyrosine phosphatase 2C, PTP-2C, SH-PTP2, SHP-2, Shp2, SH-PTP3, PTPN11, PTP2C, SHPTP2
Target/Specificity This SHP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 526-551 amino acids from human SHP2.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSHP2 Antibody (Y546) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PTPN11
Synonyms PTP2C, SHPTP2
Function Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus (PubMed:10655584, PubMed:18559669, PubMed:18829466, PubMed:26742426, PubMed:28074573). Positively regulates MAPK signal transduction pathway (PubMed:28074573). Dephosphorylates GAB1, ARHGAP35 and EGFR (PubMed:28074573). Dephosphorylates ROCK2 at 'Tyr-722' resulting in stimulation of its RhoA binding activity (PubMed:18559669). Dephosphorylates CDC73 (PubMed:26742426). Dephosphorylates SOX9 on tyrosine residues, leading to inactivate SOX9 and promote ossification (By similarity).
Cellular Location Cytoplasm. Nucleus
Tissue Location Widely expressed, with highest levels in heart, brain, and skeletal muscle.
Research Areas
Citations ( 0 )

Background

SHP2, also known as PTPN11, is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains two tandem Src homology-2 domains, which function as phospho-tyrosine binding domains and mediate the interaction of this PTP with its substrates. This PTP is widely expressed in most tissues and plays a regulatory role in various cell signaling events that are important for a diversity of cell functions, such as mitogenic activation, metabolic control, transcription regulation, and cell migration. Mutations in the gene are a cause of Noonan syndrome as well as acute myeloid leukemia.

References

Chan, R.J., et al., Blood 105(9):3737-3742 (2005).
Sturla, L.M., et al., J. Biol. Chem. 280(15):14597-14604 (2005).
Loh, M.L., et al., Leuk. Res. 29(4):459-462 (2005).
Wang, Q., et al., J. Biol. Chem. 280(9):8397-8406 (2005).
Niihori, T., et al., J. Hum. Genet. 50(4):192-202 (2005).

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$ 162.50
$ 54.50
Cat# AP8471e
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