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PLA2G4A Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

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  • IF - PLA2G4A Antibody (Center) AP8510c
    Confocal immunofluorescent analysis of PLA2G4A Antibody (Center)(Cat. #AP8510c) with NCI-H460 cell followed by Alexa Fluor搴?488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
  • WB - PLA2G4A Antibody (Center) AP8510c
    Western blot analysis of lysate from 293 cell line, using PLA2G4A Antibody (Center) (Cat. #AP8510c). AP8510c was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
  • IHC-P - PLA2G4A Antibody (Center) AP8510c
    Formalin-fixed and paraffin-embedded human lung carcinoma with PLA2G4A Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - PLA2G4A Antibody (Center) AP8510c
    Flow cytometric analysis of NCI-H292 cells using PLA2G4A Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P47712
Other Accession P50393, Q9TT38, P47713, P50392, P49147, O77793
Reactivity Human
Predicted Chicken, Zebrafish, Horse, Mouse, Rabbit, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 85239 Da
Antigen Region 513-541 aa
Additional Information
Gene ID 5321
Other Names Cytosolic phospholipase A2, cPLA2, Phospholipase A2 group IVA, Phospholipase A2, Phosphatidylcholine 2-acylhydrolase, Lysophospholipase, PLA2G4A, CPLA2, PLA2G4
Target/Specificity This PLA2G4A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 513-541 amino acids from the Central region of human PLA2G4A.
Dilution IF~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPLA2G4A Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PLA2G4A
Synonyms CPLA2, PLA2G4
Function Has primarily calcium-dependent phospholipase and lysophospholipase activities, with a major role in membrane lipid remodeling and biosynthesis of lipid mediators of the inflammatory response (PubMed:7794891, PubMed:8619991, PubMed:8702602, PubMed:9425121, PubMed:10358058, PubMed:14709560, PubMed:16617059, PubMed:17472963, PubMed:27642067, PubMed:18451993). Plays an important role in embryo implantation and parturition through its ability to trigger prostanoid production (By similarity). Preferentially hydrolyzes the ester bond of the fatty acyl group attached at sn-2 position of phospholipids (phospholipase A2 activity) (PubMed:7794891, PubMed:8619991, PubMed:9425121, PubMed:10358058, PubMed:17472963, PubMed:18451993). Selectively hydrolyzes sn-2 arachidonoyl group from membrane phospholipids, providing the precursor for eicosanoid biosynthesis via the cyclooxygenase pathway (PubMed:18451993, PubMed:7794891, PubMed:9425121, PubMed:10358058, PubMed:17472963). In an alternative pathway of eicosanoid biosynthesis, hydrolyzes sn-2 fatty acyl chain of eicosanoid lysophopholipids to release free bioactive eicosanoids (PubMed:27642067). Hydrolyzes the ester bond of the fatty acyl group attached at sn-1 position of phospholipids (phospholipase A1 activity) only if an ether linkage rather than an ester linkage is present at the sn-2 position. This hydrolysis is not stereospecific (PubMed:7794891). Has calcium-independent phospholipase A2 and lysophospholipase activities in the presence of phosphoinositides (PubMed:12672805). Has O-acyltransferase activity. Catalyzes the transfer of fatty acyl chains from phospholipids to a primary hydroxyl group of glycerol (sn-1 or sn-3), potentially contributing to monoacylglycerol synthesis (PubMed:7794891).
Cellular Location Cytoplasm. Golgi apparatus membrane. Nucleus envelope Note=Translocates to intracellular membranes in a calcium-dependent way.
Tissue Location Expressed in various cells and tissues such as macrophages, neutrophils, fibroblasts and lung endothelium. Expressed in platelets (at protein level) (PubMed:25102815)
Research Areas
Citations (0)

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PLA2G4A is a member of the cytosolic phospholipase A2 group IV family. The enzyme catalyzes the hydrolysis of membrane phospholipids to release arachidonic acid which is subsequently metabolized into eicosanoids. Eicosanoids, including prostaglandins and leukotrienes, are lipid-based cellular hormones that regulate hemodynamics, inflammatory responses, and other intracellular pathways. The hydrolysis reaction also produces lysophospholipids that are converted into platelet-activating factor. The enzyme is activated by increased intracellular Ca(2+) levels and phosphorylation, resulting in its translocation from the cytosol and nucleus to perinuclear membrane vesicles.


Sharp,J.D.,, J. Biol. Chem. 266 (23), 14850-14853 (1991)
Clark,J.D.,, Cell 65 (6), 1043-1051 (1991)

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$ 325.00
$ 109.00
Cat# AP8510c
Availability: In Stock
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