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HLA-E Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - HLA-E Antibody (Center) AP8517C
    Western blot analysis of HLA-E Antibody (Center) (Cat. #AP8517c) in MDA-MB231 cell line lysates (35ug/lane). HLA-E (arrow) was detected using the purified Pab.
  • WB - HLA-E Antibody (Center) AP8517C
    Western blot analysis of HLA-E (arrow) using rabbit polyclonal HLA-E Antibody (Center) (Cat. #AP8517c). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HLA-E gene.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P13747
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 40058 Da
Antigen Region 108-135 aa
Additional Information
Gene ID 3133
Other Names HLA class I histocompatibility antigen, alpha chain E, MHC class I antigen E, HLA-E, HLA-62, HLAE
Target/Specificity This HLA-E antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 108-135 amino acids from the Central region of human HLA-E.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHLA-E Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HLA-E {ECO:0000303|PubMed:9486650, ECO:0000312|HGNC:HGNC:4962}
Function Non-classical major histocompatibility class Ib molecule involved in immune self-nonself discrimination. In complex with B2M/beta-2-microglobulin binds nonamer self-peptides derived from the signal sequence of classical MHC class Ia molecules (VL9 peptides) (PubMed:9754572, PubMed:18083576, PubMed:18339401). Peptide-bound HLA- E-B2M heterotrimeric complex primarily functions as a ligand for natural killer (NK) cell inhibitory receptor KLRD1-KLRC1, enabling NK cells to monitor the expression of other MHC class I molecules in healthy cells and to tolerate self (PubMed:9754572, PubMed:9486650, PubMed:17179229, PubMed:18083576). Upon cellular stress, preferentially binds signal sequence-derived peptides from stress-induced chaperones and is no longer recognized by NK cell inhibitory receptor KLRD1-KLRC1, resulting in impaired protection from NK cells (PubMed:12461076). Binds signal sequence-derived peptides from non-classical MHC class Ib HLA-G molecules and acts as a ligand for NK cell activating receptor KLRD1- KLRC2, likely playing a role in the generation and effector functions of adaptive NK cells and in maternal-fetal tolerance during pregnancy (PubMed:9754572, PubMed:30134159). Besides self-peptides, can also bind and present pathogen-derived peptides conformationally similar to VL9 peptides to alpha-beta T cell receptor (TCR) on unconventional CD8+ cytotoxic T cells, ultimately triggering antimicrobial immune response (PubMed:16474394, PubMed:30087334).
Cellular Location Cell membrane; Single-pass type I membrane protein. Golgi apparatus membrane
Tissue Location Expressed in secretory endometrial cells during pregnancy (at protein level). The expression in nonlymphoid tissues is restricted to endothelial cells from all types of vessels, including arteries, veins, capillaries, and lymphatics (at protein level). In lymphoid organs, it is mainly expressed in endothelial venules, B and T cells, monocytes, macrophages, NK cells and megakaryocytes (at protein level).
Research Areas
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Background

HLA-E preferably binds to a peptide derived from the signal sequence of most HLA-A, -B, -C and -G molecules.

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$ 162.50
$ 54.50
Cat# AP8517C
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