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OLIG3 Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - OLIG3 Antibody (Center) AP8632c
    Western blot analysis of OLIG3 Antibody (Center) (Cat. #AP8632c) in mouse spleen tissue lysates (35ug/lane). OLIG3 (arrow) was detected using the purified Pab.
    detail
  • IHC-P - OLIG3 Antibody (Center) AP8632c
    Formalin-fixed and paraffin-embedded human brain tissue with OLIG3 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
  • FC - OLIG3 Antibody (Center) AP8632c
    OLIG3 Antibody (Center) (Cat. #AP8632c) flow cytometric analysis of U251 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, IHC-P, WB, E
Primary Accession Q7RTU3
Other Accession Q6PFG8
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 29358 Da
Antigen Region 74-100 aa
Additional Information
Gene ID 167826
Other Names Oligodendrocyte transcription factor 3, Oligo3, Class B basic helix-loop-helix protein 7, bHLHb7, Class E basic helix-loop-helix protein 20, bHLHe20, OLIG3, BHLHB7, BHLHE20
Target/Specificity This OLIG3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 74-100 amino acids from the Central region of human OLIG3.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsOLIG3 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name OLIG3
Synonyms BHLHB7, BHLHE20
Function May determine the distinct specification program of class A neurons in the dorsal part of the spinal cord and suppress specification of class B neurons.
Cellular Location Nucleus {ECO:0000255|PROSITE-ProRule:PRU00981}.
Research Areas
Citations (0)
citation

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Background

OLIG3 may determine the distinct specification program of class A neurons in the dorsal part of the spinal cord and suppress specification of class B neurons.

References

Muller,T., et.al., Genes Dev. 19 (6), 733-743 (2005)

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$ 182.50
$ 70.00
Cat# AP8632c
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