|Application ||WB, FC, IHC-P, E|
|Other Accession||P07896, Q9DBM2|
|Calculated MW||79495 Da|
|Antigen Region||662-690 aa|
|Other Names||Peroxisomal bifunctional enzyme, PBE, PBFE, Enoyl-CoA hydratase/3, 2-trans-enoyl-CoA isomerase, 3-hydroxyacyl-CoA dehydrogenase, EHHADH, ECHD|
|Target/Specificity||This EHHADH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 662-690 amino acids from the C-terminal region of human EHHADH.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||EHHADH Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Peroxisomal trifunctional enzyme possessing 2-enoyl-CoA hydratase, 3-hydroxyacyl-CoA dehydrogenase, and delta 3, delta 2-enoyl- CoA isomerase activities. Catalyzes two of the four reactions of the long straight chain fatty acids peroxisomal beta-oxidation pathway. Optimal isomerase for 2,5 double bonds into 3,5 form isomerization in a range of enoyl-CoA species (Probable). Also able to isomerize both 3- cis and 3-trans double bonds into the 2-trans form in a range of enoyl- CoA species (By similarity). With HSD17B4, catalyzes the hydration of trans-2-enoyl-CoA and the dehydrogenation of 3-hydroxyacyl-CoA, but with opposite chiral specificity (PubMed:15060085). Regulates the amount of medium-chain dicarboxylic fatty acids which are essential regulators of all fatty acid oxidation pathways (By similarity). Also involved in the degradation of long-chain dicarboxylic acids through peroxisomal beta-oxidation (PubMed:15060085).|
|Tissue Location||Liver and kidney. Strongly expressed in the terminal segments of the proximal tubule. Lower amounts seen in the brain.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
email@example.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
EHHADH is a bifunctional enzyme and is one of the four enzymes of the peroxisomal beta-oxidation pathway. The N-terminal region of the encoded protein contains enoyl-CoA hydratase activity while the C-terminal region contains 3-hydroxyacyl-CoA dehydrogenase activity.
Chen,G.L., et.al., Biochem. Biophys. Res. Commun. 178 (3), 1084-1091 (1991)
Lu,Y., et.al., J. Lipid Res. 49 (12), 2582-2589 (2008)
If you have used an Abcepta product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at firstname.lastname@example.org.