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ICAM1 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - ICAM1 Antibody (C-term) AP8656b
    Western blot analysis of ICAM1 (arrow) using rabbit polyclonal ICAM1 Antibody (C-term) (Cat. #AP8656b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ICAM1 gene (Lane 2).
  • FC - ICAM1 Antibody (C-term) AP8656b
    ICAM1 Antibody (C-term) (Cat. #AP8656b) flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • SPECIFICATION
  • CITATIONS: 1
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, WB, E
Primary Accession P05362
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 57825 Da
Antigen Region 488-514 aa
Additional Information
Gene ID 3383
Other Names Intercellular adhesion molecule 1, ICAM-1, Major group rhinovirus receptor, CD54, ICAM1
Target/Specificity This ICAM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 488-514 amino acids from the C-terminal region of human ICAM1.
Dilution WB~~1:1000
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsICAM1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ICAM1
Function ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation.
Cellular Location Membrane; Single-pass type I membrane protein.
Research Areas
Citations ( 0 )

Background

ICAM1 is a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor.

References

Denkers,I.A., et.al., Leuk. Res. 16 (5), 469-474 (1992)
Rossler,K., et.al., J. Neurosci. Res. 31 (2), 365-374 (1992)

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$ 162.50
$ 54.50
Cat# AP8656b
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