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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   PHYH Antibody (N-term)   

PHYH Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - PHYH Antibody (N-term) AP8711a
    Western blot analysis of PHYH Antibody (N-term) (Cat. #AP8711a) in MDA-MB435 cell line lysates (35ug/lane). PHYH (arrow) was detected using the purified Pab.
  • WB - PHYH Antibody (N-term) AP8711a
    Western blot analysis of PHYH (arrow) using rabbit polyclonal PHYH Antibody (N-term) (Cat. #AP8711a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PHYH gene.
  • IHC-P - PHYH Antibody (N-term) AP8711a
    Formalin-fixed and paraffin-embedded human skeletal muscle reacted with PHYH Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - PHYH Antibody (N-term) AP8711a
    PHYH Antibody (N-term) (Cat. #AP8711a) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O14832
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 38538 Da
Antigen Region 59-87 aa
Additional Information
Gene ID 5264
Other Names Phytanoyl-CoA dioxygenase, peroxisomal, Phytanic acid oxidase, Phytanoyl-CoA alpha-hydroxylase, PhyH, PHYH, PAHX
Target/Specificity This PHYH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 59-87 amino acids from the N-terminal region of human PHYH.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPHYH Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms PAHX
Function Catalyzes the 2-hydroxylation of not only racemic phytanoyl- CoA and the isomers of 3-methylhexadecanoyl-CoA, but also a variety of other mono-branched 3-methylacyl-CoA esters (with a chain length of at least seven carbon atoms) and straight-chain acyl-CoA esters (with a chain length longer than four carbon atoms) (PubMed:9326939, PubMed:10744784, PubMed:12031666, PubMed:12923223). Does not hydroxylate long and very long straight chain acyl-CoAs or 2- methyl- and 4-methyl-branched acyl-CoAs (PubMed:10744784, PubMed:12923223).
Cellular Location Peroxisome.
Tissue Location Expressed in liver, kidney, and T-cells, but not in spleen, brain, heart, lung and skeletal muscle
Research Areas
Citations (0)

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PHYH2 catalyzes a carbon-carbon cleavage reaction; cleaves a 2-hydroxy-3-methylacyl-CoA into formyl-CoA and a 2-methyl-branched fatty aldehyde.


Foulon,V.,, J. Biol. Chem. 280 (11), 9802-9812 (2005)
Kikuchi,M.,, J. Biol. Chem. 279 (1), 421-428 (2004)

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$ 162.50
$ 54.50
Cat# AP8711a
Availability: In Stock
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