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BDK_1 Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - BDK_1 Antibody (Center) AP8735C
    All lanes : Anti-BDK_1 Antibody (Center) at 1:1000 dilution Lane 1: HepG2 whole cell lysate Lane 2: Hela whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 40 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • FC - BDK_1 Antibody (Center) AP8735C
    BDKRB1 Antibody (Center) (Cat. #AP8735c) flow cytometric analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, FC, E
Primary Accession P46663
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 40495 Da
Antigen Region 213-239 aa
Additional Information
Gene ID 623
Other Names B1 bradykinin receptor, B1R, BK-1 receptor, BDKRB1, BRADYB1
Target/Specificity This BDK_1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 213-239 amino acids from the Central region of human BDK_1.
Dilution WB~~1:1000
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsBDK_1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name BDKRB1
Synonyms BRADYB1
Function This is a receptor for bradykinin. Could be a factor in chronic pain and inflammation.
Cellular Location Cell membrane; Multi-pass membrane protein
Research Areas
Citations (0)
citation

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Background

Bradykinin, a 9 aa peptide, is generated in pathophysiologic conditions such as inflammation, trauma, burns, shock, and allergy. Two types of G-protein coupled receptors have been found which bind bradykinin and mediate responses to these pathophysiologic conditions. BDKRB1 is one of these receptors and is synthesized de novo following tissue injury. Receptor binding leads to an increase in the cytosolic calcium ion concentration, ultimately resulting in chronic and acute inflammatory responses.

References

Bachvarov,D.R., et.al., Genomics 33 (3), 374-381 (1996)

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$ 365.00
$ 140.00
Cat# AP8735C
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