|Application ||FC, WB, E|
|Other Accession||P79384, Q99MN9, Q2TBR0|
|Predicted||Bovine, Mouse, Pig|
|Calculated MW||58216 Da|
|Antigen Region||82-107 aa|
|Other Names||Propionyl-CoA carboxylase beta chain, mitochondrial, PCCase subunit beta, Propanoyl-CoA:carbon dioxide ligase subunit beta, PCCB|
|Target/Specificity||This PCCB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 82-107 amino acids from the Central region of human PCCB.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||PCCB Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||This is one of the 2 subunits of the biotin-dependent propionyl-CoA carboxylase (PCC), a mitochondrial enzyme involved in the catabolism of odd chain fatty acids, branched-chain amino acids isoleucine, threonine, methionine, and valine and other metabolites (PubMed:6765947, PubMed:15890657). Propionyl-CoA carboxylase catalyzes the carboxylation of propionyl-CoA/propanoyl-CoA to D-methylmalonyl- CoA/(S)-methylmalonyl-CoA (PubMed:6765947, PubMed:15890657). Within the holoenzyme, the alpha subunit catalyzes the ATP-dependent carboxylation of the biotin carried by the biotin carboxyl carrier (BCC) domain, while the beta subunit then transfers the carboxyl group from carboxylated biotin to propionyl-CoA (By similarity). Propionyl-CoA carboxylase also significantly acts on butyryl-CoA/butanoyl-CoA, which is converted to ethylmalonyl-CoA/(2S)-ethylmalonyl-CoA at a much lower rate (PubMed:6765947). Other alternative minor substrates include (2E)- butenoyl-CoA/crotonoyl-CoA (By similarity).|
|Cellular Location||Mitochondrion matrix|
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Provided below are standard protocols that you may find useful for product applications.
PCCB is a subunit of the propionyl-CoA carboxylase (PCC) enzyme, which is involved in the catabolism of propionyl-CoA. PCC is a mitochondrial enzyme that probably acts as a dodecamer of six alpha subunits and six beta subunits.
Yang,X., et.al.,Mol. Genet. Metab. 81 (4), 335-342 (2004)
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