PCCB Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| FC, WB, E |
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Primary Accession | P05166 |
Other Accession | P79384, Q99MN9, Q2TBR0 |
Reactivity | Human |
Predicted | Bovine, Mouse, Pig |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 58216 Da |
Antigen Region | 82-107 aa |
Gene ID | 5096 |
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Other Names | Propionyl-CoA carboxylase beta chain, mitochondrial, PCCase subunit beta, Propanoyl-CoA:carbon dioxide ligase subunit beta, PCCB |
Target/Specificity | This PCCB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 82-107 amino acids from the Central region of human PCCB. |
Dilution | WB~~1:1000 FC~~1:10~50 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | PCCB Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | PCCB (HGNC:8654) |
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Function | This is one of the 2 subunits of the biotin-dependent propionyl-CoA carboxylase (PCC), a mitochondrial enzyme involved in the catabolism of odd chain fatty acids, branched-chain amino acids isoleucine, threonine, methionine, and valine and other metabolites (PubMed:6765947, PubMed:15890657). Propionyl-CoA carboxylase catalyzes the carboxylation of propionyl-CoA/propanoyl-CoA to D-methylmalonyl- CoA/(S)-methylmalonyl-CoA (PubMed:6765947, PubMed:15890657). Within the holoenzyme, the alpha subunit catalyzes the ATP-dependent carboxylation of the biotin carried by the biotin carboxyl carrier (BCC) domain, while the beta subunit then transfers the carboxyl group from carboxylated biotin to propionyl-CoA (By similarity). Propionyl-CoA carboxylase also significantly acts on butyryl-CoA/butanoyl-CoA, which is converted to ethylmalonyl-CoA/(2S)-ethylmalonyl-CoA at a much lower rate (PubMed:6765947). Other alternative minor substrates include (2E)- butenoyl-CoA/crotonoyl-CoA (By similarity). |
Cellular Location | Mitochondrion matrix |
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Provided below are standard protocols that you may find useful for product applications.
Background
PCCB is a subunit of the propionyl-CoA carboxylase (PCC) enzyme, which is involved in the catabolism of propionyl-CoA. PCC is a mitochondrial enzyme that probably acts as a dodecamer of six alpha subunits and six beta subunits.
References
Yang,X., et.al.,Mol. Genet. Metab. 81 (4), 335-342 (2004)
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