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ATF7 Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • IF - ATF7 Antibody (N-term) AP8844a
    Fluorescent confocal image of Hela cell stained with ATF7 Antibody (N-term)(Cat#AP8844a).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ATF7 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).ATF7 immunoreactivity is localized to Nucleus and Cytoplasm significantly.
    detail
  • WB - ATF7 Antibody (N-term) AP8844a
    ATF7 Antibody (N-term) (Cat. #AP8844a) western blot analysis in huamn placenta tissue lysates (35ug/lane).This demonstrates the ATF7 antibody detected the ATF7 protein (arrow).
    detail
  • FC - ATF7 Antibody (N-term) AP8844a
    ATF7 Antibody (N-term) (Cat. #AP8844a) flow cytometric analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF, FC, E
Primary Accession P17544
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 51757 Da
Antigen Region 68-96 aa
Additional Information
Gene ID 11016
Other Names Cyclic AMP-dependent transcription factor ATF-7, cAMP-dependent transcription factor ATF-7, Activating transcription factor 7, Transcription factor ATF-A, ATF7, ATFA
Target/Specificity This ATF7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 68-96 amino acids from the N-terminal region of human ATF7.
Dilution IF~~1:10~50
WB~~1:1000
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsATF7 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ATF7
Synonyms ATFA
Function Stress-responsive chromatin regulator that plays a role in various biological processes including innate immunological memory, adipocyte differentiation or telomerase regulation (PubMed:29490055). In absence of stress, contributes to the formation of heterochromatin and heterochromatin-like structure by recruiting histone H3K9 tri- and di-methyltransferases thus silencing the transcription of target genes such as STAT1 in adipocytes, or genes involved in innate immunity in macrophages and adipocytes (By similarity). Stress induces ATF7 phosphorylation that disrupts interactions with histone methyltransferase and enhances the association with coactivators containing histone acetyltransferase and/or histone demethylase, leading to disruption of the heterochromatin-like structure and subsequently transcriptional activation (By similarity). In response to TNF-alpha, which is induced by various stresses, phosphorylated ATF7 and telomerase are released from telomeres leading to telomere shortening (PubMed:29490055). Also plays a role in maintaining epithelial regenerative capacity and protecting against cell death during intestinal epithelial damage and repair (By similarity).
Cellular Location Nucleus {ECO:0000255|PROSITE-ProRule:PRU00978, ECO:0000269|PubMed:17264123}. Nucleus, nucleoplasm. Chromosome, telomere. Note=Mainly nucleoplasmic. Restricted distribution to the perinuculear region. The sumoylated form locates to the nuclear periphery
Tissue Location Expressed in various tissues including heart, brain, placenta, lung and skeletal muscle. Highest levels in skeletal muscle. Lowest in lung and placenta.
Research Areas
Citations (0)
citation

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Background

The cyclic AMP-dependent transcription factor ATF7 is a member of the bZIP family of proteins and is known to bind to the cAMP response element (CRE) as a homodimer.

References

Hamard,P.J.,et.al., Oncogene 24 (21), 3472-3483 (2005)

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$ 365.00
$ 140.00
Cat# AP8844a
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