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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   CYP51A1 Antibody (C-term)   

CYP51A1 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - CYP51A1 Antibody (C-term) AP8874b
    Western blot analysis of CYP51A1 Antibody (C-term) (Cat. #AP8874b) in HL-60 cell line lysates (35ug/lane). CYP51A1 (arrow) was detected using the purified Pab.
  • IHC-P - CYP51A1 Antibody (C-term) AP8874b
    Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with CYP51A1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB, E
Primary Accession Q16850
Other Accession Q64654, O46420, Q8K0C4, Q4R8S6
Reactivity Human
Predicted Monkey, Mouse, Pig, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 56806 Da
Antigen Region 474-503 aa
Additional Information
Gene ID 1595
Other Names Lanosterol 14-alpha demethylase, LDM, CYPLI, Cytochrome P450 51A1, Cytochrome P450-14DM, Cytochrome P45014DM, Cytochrome P450LI, Sterol 14-alpha demethylase, CYP51A1, CYP51
Target/Specificity This CYP51A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 474-503 amino acids from the C-terminal region of human CYP51A1.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCYP51A1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CYP51A1
Synonyms CYP51
Function A cytochrome P450 monooxygenase involved in sterol biosynthesis. Catalyzes 14-alpha demethylation of lanosterol and 24,25- dihydrolanosterol likely through sequential oxidative conversion of 14- alpha methyl group to hydroxymethyl, then to carboxylaldehyde, followed by the formation of the delta 14,15 double bond in the sterol core and concomitant release of formic acid (PubMed:20149798, PubMed:8619637). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH- ferrihemoprotein reductase) (PubMed:20149798, PubMed:8619637).
Cellular Location Endoplasmic reticulum membrane {ECO:0000250|UniProtKB:Q64654}; Single-pass membrane protein. Microsome membrane {ECO:0000250|UniProtKB:Q64654}; Single-pass membrane protein
Tissue Location Ubiquitously expressed with highest levels in testis, ovary, adrenal, prostate, liver, kidney and lung
Research Areas
Citations (0)

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Background

CYP51A1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum protein participates in the synthesis of cholesterol by catalyzing the removal of the 14alpha-methyl group from lanosterol.

References

Wang,Y., et.al., J. Biol. Chem. 283 (39), 26332-26339 (2008)

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Cat# AP8874b
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