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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   GAL3ST1 Antibody (Center)   

GAL3ST1 Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - GAL3ST1 Antibody (Center) AP8884c
    Western blot analysis of GAL3ST1 Antibody (Center) (Cat. #AP8884c) in mouse cerebellum tissue lysates (35ug/lane). GAL3ST1 (arrow) was detected using the purified Pab.
    detail
  • WB - GAL3ST1 Antibody (Center) AP8884c
    All lanes : Anti-GAL3ST1 Antibody (Center) at 1:2000 dilution Lane 1: Human kidney lysate Lane 2: Mouse kidney lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 49 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IHC-P - GAL3ST1 Antibody (Center) AP8884c
    Formalin-fixed and paraffin-embedded human brain tissue reacted with GAL3ST1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
  • FC - GAL3ST1 Antibody (Center) AP8884c
    GAL3ST1 Antibody (Center) (Cat. #AP8884c) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, WB, IHC-P, E
Primary Accession Q99999
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 48764 Da
Antigen Region 88-116 aa
Additional Information
Gene ID 9514
Other Names Galactosylceramide sulfotransferase, GalCer sulfotransferase, 3'-phosphoadenosine-5'-phosphosulfate:GalCer sulfotransferase, 3'-phosphoadenylylsulfate:galactosylceramide 3'-sulfotransferase, Cerebroside sulfotransferase, GAL3ST1, CST
Target/Specificity This GAL3ST1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 88-116 amino acids from the Central region of human GAL3ST1.
Dilution WB~~1:2000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGAL3ST1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GAL3ST1 (HGNC:24240)
Function Catalyzes the transfer of a sulfate group to position 3 of non-reducing beta-galactosyl residues in glycerolipids and sphingolipids, therefore participates in the biosynthesis of sulfoglycolipids (PubMed:9030544, PubMed:8830034). Catalyzes the synthesis of galactosylceramide sulfate (sulfatide), a major lipid component of the myelin sheath and of monogalactosylalkylacylglycerol sulfate (seminolipid), present in spermatocytes (PubMed:8830034). Seems to prefer beta-glycosides at the non-reducing termini of sugar chains attached to a lipid moiety (PubMed:8830034). Also acts on lactosylceramide, galactosyl 1-alkyl-2-sn-glycerol and galactosyl diacylglycerol (in vitro) (PubMed:8830034).
Cellular Location Golgi apparatus membrane; Single- pass type II membrane protein
Tissue Location Expressed in kidney proximal tubule, gastric mucosa and adenocarcinoma (PubMed:9030544, PubMed:10785389). Highly expressed in renal cell carcinoma cell lines (PubMed:9030544, PubMed:8830034)
Research Areas
Citations (0)
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Background

Sulfonation, an important step in the metabolism of many drugs, xenobiotics, hormones, and neurotransmitters, is catalyzed by sulfotransferases. GAL3ST1 is galactosylceramide sulfotransferase which catalyzes the conversion between 3'-phosphoadenylylsulfate + a galactosylceramide to adenosine 3',5'-bisphosphate + galactosylceramide sulfate. Activity of this sulfotransferase is enhanced in renal cell carcinoma.

References

Siegrist,H.P., et.al., Biochim. Biophys. Acta 489 (1), 58-63 (1977)
Stein,C., et.al., J. Biol. Chem. 264 (2), 1252-1259 (1989)

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$ 182.50
$ 70.00
Cat# AP8884c
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