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GSTA4 Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - GSTA4 Antibody (N-term) AP8970a
    Western blot analysis of GSTA4 Antibody (N-term) (Cat. #AP8970a) in HepG2 cell line lysates (35ug/lane). GSTA4 (arrow) was detected using the purified Pab.
    detail
  • IHC-P - GSTA4 Antibody (N-term) AP8970a
    Formalin-fixed and paraffin-embedded human brain tissue reacted with GSTA4 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
  • FC - GSTA4 Antibody (N-term) AP8970a
    GSTA4 Antibody (N-term) (Cat. #AP8970a) flow cytometric analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, IHC-P, WB, E
Primary Accession O15217
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 25704 Da
Antigen Region 62-90 aa
Additional Information
Gene ID 2941
Other Names Glutathione S-transferase A4, GST class-alpha member 4, Glutathione S-transferase A4-4, GSTA4
Target/Specificity This GSTA4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 62-90 amino acids from the N-terminal region of human GSTA4.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGSTA4 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GSTA4
Function Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. This isozyme has a high catalytic efficiency with 4-hydroxyalkenals such as 4- hydroxynonenal (4-HNE).
Cellular Location Cytoplasm.
Tissue Location Expressed at a high level in brain, placenta, and skeletal muscle and much lower in lung and liver
Research Areas
Citations (0)
citation

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Background

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. This isozyme has a high catalytic efficiency with 4-hydroxyalkenals such as 4-hydroxynonenal (4-HNE).

References

Bruns C.M.,et.al., J. Mol. Biol. 288:427-439(1999).

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$ 182.50
$ 70.00
Cat# AP8970a
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