- CITATIONS: 4
|Application ||FC, IHC-P, WB, E|
|Calculated MW||35386 Da|
|Antigen Region||26-55 aa|
|Other Names||Secreted frizzled-related protein 1, FRP-1, sFRP-1, Secreted apoptosis-related protein 2, SARP-2, SFRP1, FRP, FRP1, SARP2|
|Target/Specificity||This SFRP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 26-55 amino acids from the N-terminal region of human SFRP1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||SFRP1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||FRP, FRP1, SARP2|
|Function||Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP1 decreases intracellular beta-catenin levels (By similarity). Has antiproliferative effects on vascular cells, in vitro and in vivo, and can induce, in vivo, an angiogenic response. In vascular cell cycle, delays the G1 phase and entry into the S phase (By similarity). In kidney development, inhibits tubule formation and bud growth in metanephroi (By similarity). Inhibits WNT1/WNT4-mediated TCF- dependent transcription.|
|Cellular Location||Secreted. Note=Cell membrane or extracellular matrix-associated. Released by heparin-binding|
|Tissue Location||Widely expressed. Absent from lung, liver and peripheral blood leukocytes. Highest levels in heart and fetal kidney Also expressed in testis, ovary, fetal brain and lung, leiomyomal cells, myometrial cells and vascular smooth muscle cells. Expressed in foreskin fibroblasts and in keratinocytes|
Provided below are standard protocols that you may find useful for product applications.
SFRP1 encodes a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. Members of this family act as soluble modulators of Wnt signaling; epigenetic silencing of SFRP genes leads to deregulated activation of the Wnt-pathway which is associated with cancer.
Huang,D., et.al., J. Cancer Res. Clin. Oncol. 136 (3), 395-401 (2010);
Yang,Z.Q., et.al., Int. J. Cancer 125 (7), 1613-1621 (2009).
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