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eIF5A Antibody
Rabbit mAb
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Application 
| WB, IHC, FC, ICC, IP |
|---|---|
| Primary Accession | P63241 |
| Reactivity | Rat |
| Clonality | Monoclonal |
| Other Names | EIF-5A; EIF5A1; eIF5AI; Eukaryotic initiation factor 5A; |
| Isotype | Rabbit IgG |
| Host | Rabbit |
| Calculated MW | 16832 Da |
| Dilution | WB 1:5000~1:10000 IHC 1:50~1:200 ICC/IF 1:100~1:500 IP 1:50 FC 1:50 |
|---|---|
| Purification | Affinity-chromatography |
| Immunogen | A synthesized peptide derived from human eIF5A |
| Description | mRNA-binding protein involved in translation elongation. Has an important function at the level of mRNA turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. With syntenin SDCBP, functions as a regulator of p53/TP53 and p53/TP53-dependent apoptosis. Regulates also TNF-alpha-mediated apoptosis. |
| Storage Condition and Buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle. |
| Name | EIF5A (HGNC:3300) |
|---|---|
| Function | Translation factor that promotes translation elongation and termination, particularly upon ribosome stalling at specific amino acid sequence contexts (PubMed:33547280). Binds between the exit (E) and peptidyl (P) site of the ribosome and promotes rescue of stalled ribosome: specifically required for efficient translation of polyproline-containing peptides as well as other motifs that stall the ribosome (By similarity). Acts as a ribosome quality control (RQC) cofactor by joining the RQC complex to facilitate peptidyl transfer during CAT tailing step (By similarity). Also involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity (PubMed:16987817). With syntenin SDCBP, functions as a regulator of p53/TP53 and p53/TP53-dependent apoptosis (PubMed:15371445). Also regulates TNF-alpha-mediated apoptosis (PubMed:15452064, PubMed:17187778). Mediates effects of polyamines on neuronal process extension and survival (PubMed:17360499). Is required for autophagy by assisting the ribosome in translating the ATG3 protein at a specific amino acid sequence, the 'ASP-ASP-Gly' motif, leading to the increase of the efficiency of ATG3 translation and facilitation of LC3B lipidation and autophagosome formation (PubMed:29712776). |
| Cellular Location | Cytoplasm. Nucleus. Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side. Note=Hypusine modification promotes the nuclear export and cytoplasmic localization and there was a dynamic shift in the localization from predominantly cytoplasmic to primarily nuclear under apoptotic inducing conditions (PubMed:19379712, PubMed:27306458). Nuclear export of hypusinated protein is mediated by XPO4 (PubMed:10944119, PubMed:27306458). |
| Tissue Location | Expressed in umbilical vein endothelial cells and several cancer cell lines (at protein level) |
Citations (0)
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Application Protocols
Provided below are standard protocols that you may find useful for product applications.
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$ 385.00
$ 195.00
Cat# AP90640
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