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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   CDC20 Antibody (N-term)   

CDC20 Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - CDC20 Antibody (N-term) AP9138a
    Western blot analysis of CDC20 Antibody (N-term) (Cat. #AP9138a) in Hela cell line lysates (35ug/lane). CDC20 (arrow) was detected using the purified Pab.
    detail
  • WB - CDC20 Antibody (N-term) AP9138a
    Western blot analysis of CDC20 (arrow) using rabbit polyclonal CDC20 Antibody (N-term) (Cat. #AP9138a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CDC20 gene.
    detail
  • FC - CDC20 Antibody (N-term) AP9138a
    CDC20 Antibody (N-term) (Cat. #AP9138a) flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, FC, E
Primary Accession Q12834
Other Accession Q5H7C0
Reactivity Human
Predicted Pig
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 54723 Da
Antigen Region 57-84 aa
Additional Information
Gene ID 991
Other Names Cell division cycle protein 20 homolog, p55CDC, CDC20
Target/Specificity This CDC20 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 57-84 amino acids from the N-terminal region of human CDC20.
Dilution WB~~1:1000
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCDC20 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CDC20
Function Involved in the metaphase/anaphase transition of cell cycle (PubMed:32666501). Required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and may confer substrate specificity upon the complex. Is regulated by MAD2L1: in metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates. The CDC20-APC/C complex positively regulates the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons. CDC20-APC/C-induced degradation of NEUROD2 induces presynaptic differentiation. The CDC20-APC/C complex promotes proper dilation formation and radial migration by degrading CCDC41 (By similarity).
Cellular Location Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle pole
Research Areas
Citations (0)
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Background

CDC20 is an activating regulatory factor for the APC/C (anaphase promoting complex/cyclosome). It activates the ubiquitination activity of the APC/C. CDC20 confers a strict destruction-box (D-box) dependence on APC. Levels of CDC20, as well as its binding to APC, peak in mitosis and decrease drastically at early G1.

References

Ge S., et.al., Cell Cycle 8:167-171(2009).
Gauci S., et.al., Anal. Chem. 81:4493-4501(2009).

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$ 182.50
$ 70.00
Cat# AP9138a
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