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RPL18A Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - RPL18A Antibody (C-term) AP9141b
    All lanes : Anti-RPL18A Antibody (C-term) at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: MOLT-4 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 21 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • WB - RPL18A Antibody (C-term) AP9141b
    Western blot analysis of RPL18A Antibody (C-term) (Cat. #AP9141b) in 293, mouse NIH-3T3 cell line lysates (35ug/lane). RPL18A (arrow) was detected using the purified Pab.
    detail
  • IHC-P - RPL18A Antibody (C-term) AP9141b
    Formalin-fixed and paraffin-embedded human brain tissue reacted with RPL18A Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
  • FC - RPL18A Antibody (C-term) AP9141b
    RPL18A Antibody (C-term) (Cat. #AP9141b) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, IHC-P, WB, E
Primary Accession Q02543
Other Accession P62718, P62717, Q7ZWJ4, Q3T003
Reactivity Human, Mouse
Predicted Bovine, Zebrafish, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 20762 Da
Antigen Region 140-166 aa
Additional Information
Gene ID 6142
Other Names 60S ribosomal protein L18a, RPL18A
Target/Specificity This RPL18A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 140-166 amino acids from the C-terminal region of human RPL18A.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRPL18A Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RPL18A
Function Component of the large ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell.
Cellular Location Cytoplasm.
Research Areas
Citations (0)
citation

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Background

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPL18A is a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L18AE family of ribosomal proteins. It is located in the cytoplasm.

References

Wool,I.G., et.al., Biochem. Cell Biol. 73 (11-12), 933-947 (1995)

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$ 182.50
$ 70.00
Cat# AP9141b
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