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COX5A Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - COX5A Antibody (Center) AP9154c
    Western blot analysis of COX5A Antibody (Center) (Cat. #AP9154c) in Hela cell line lysates (35ug/lane). COX5A (arrow) was detected using the purified Pab.
  • IHC-P - COX5A Antibody (Center) AP9154c
    Formalin-fixed and paraffin-embedded human brain tissue reacted with COX5A Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - COX5A Antibody (Center) AP9154c
    COX5A Antibody (Center) (Cat.#AP9154c) FC analysis of MCF-7cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, IHC-P, WB, E
Primary Accession P20674
Other Accession P11240, P12787, P00426
Reactivity Human
Predicted Bovine, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 16762 Da
Antigen Region 46-73 aa
Additional Information
Gene ID 9377
Other Names Cytochrome c oxidase subunit 5A, mitochondrial, Cytochrome c oxidase polypeptide Va, COX5A
Target/Specificity This COX5A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 46-73 amino acids from the Central region of human COX5A.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCOX5A Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name COX5A
Function Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol- cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.
Cellular Location Mitochondrion inner membrane; Peripheral membrane protein; Matrix side
Research Areas
Citations (0)

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Background

This is the heme A-containing chain of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.

References

Colinge J., et.al., Submitted (OCT-2008) to UniProtKB.

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$ 162.50
$ 54.50
Cat# AP9154c
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