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E1 Ubiquitin Activating Enzyme Antibody
Rabbit mAb
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC, FC, ICC |
|---|---|
| Primary Accession | P22314 |
| Reactivity | Rat |
| Clonality | Monoclonal |
| Other Names | A1S9; A1S9T; A1ST; AMCX1; CFAP124; GXP1; POC20; SMAX2; Uba1; UBA1A; UBE1; UBE1X; |
| Isotype | Rabbit IgG |
| Host | Rabbit |
| Calculated MW | 117849 Da |
| Dilution | WB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 FC 1:50 |
|---|---|
| Purification | Affinity-chromatography |
| Immunogen | A synthesized peptide derived from human E1 Ubiquitin Activating Enzyme |
| Description | Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thioester and free AMP. |
| Storage Condition and Buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle. |
| Name | UBA1 |
|---|---|
| Synonyms | A1S9T, UBE1 |
| Function | Catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation through the ubiquitin-proteasome system (PubMed:1447181, PubMed:1606621, PubMed:33108101). Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP (PubMed:1447181). Essential for the formation of radiation-induced foci, timely DNA repair and for response to replication stress. Promotes the recruitment of TP53BP1 and BRCA1 at DNA damage sites (PubMed:22456334). |
| Cellular Location | Cytoplasm. Mitochondrion. Nucleus [Isoform 2]: Cytoplasm |
| Tissue Location | Detected in erythrocytes (at protein level). Ubiquitous. |
Research Areas
Citations (0)
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info@abcepta.com, and receive a free "I Love Antibodies" mug.
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
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$ 195.00
$ 385.00
Cat# AP91770
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