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BEST2 Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - BEST2 Antibody (C-term) AP9246b
    Western blot analysis of BEST2 Antibody (C-term) (Cat. #AP9246b) in MDA-MB435,MDA-MB231 cell line lysates (35ug/lane).BEST2 (arrow) was detected using the purified Pab.
    detail
  • IHC-P - BEST2 Antibody (C-term) AP9246b
    Formalin-fixed and paraffin-embedded human breast carcinoma with BEST2 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB, E
Primary Accession Q8NFU1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 57139 Da
Antigen Region 374-403 aa
Additional Information
Gene ID 54831
Other Names Bestrophin-2, Vitelliform macular dystrophy 2-like protein 1, BEST2, VMD2L1
Target/Specificity This BEST2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 374-403 amino acids from the C-terminal region of human BEST2.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsBEST2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name BEST2
Synonyms VMD2L1
Function Forms calcium-sensitive chloride channels. Permeable to bicarbonate.
Cellular Location Cell membrane; Multi-pass membrane protein.
Tissue Location Mainly confined to the retinal pigment epithelium and colon.
Research Areas
Citations (0)
citation

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Background

BEST2 is a member of the bestrophin gene family of anion channels. Bestrophin genes share a similar gene structure with highly conserved exon-intron boundaries, but with distinct 3' ends. Bestrophins are transmembrane proteins that contain a homologous region rich in aromatic residues, including an invariant arg-phe-pro motif.

References

Zhang,Y., et.al, Mol. Vis. 16, 200-206 (2010)
Marsey,L.L. et.al, J. Physiol. (Lond.) 587 (PT 10), 2211-2224 (2009)

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$ 365.00
$ 140.00
Cat# AP9246b
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