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EFEMP2 Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - EFEMP2 Antibody (C-term) AP9269b
    Western blot analysis of EFEMP2 Antibody (C-term) (Cat. #AP9269b) in HepG2 cell line lysates (35ug/lane). EFEMP2 (arrow) was detected using the purified Pab;
    detail
  • WB - EFEMP2 Antibody (C-term) AP9269b
    Western blot analysis of EFEMP2 Antibody (C-term) (Cat. #AP9269b) in mouse NIH-3T3 cell line lysates (35ug/lane). EFEMP2 (arrow) was detected using the purified Pab.
    detail
  • FC - EFEMP2 Antibody (C-term) AP9269b
    EFEMP2 Antibody (C-term) (Cat. #AP9269b) flow cytometric analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, WB, E
Primary Accession O95967
Other Accession Q9WVJ9, O55058
Reactivity Human, Mouse
Predicted Hamster
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 49405 Da
Antigen Region 386-414 aa
Additional Information
Gene ID 30008
Other Names EGF-containing fibulin-like extracellular matrix protein 2, Fibulin-4, FIBL-4, Protein UPH1, EFEMP2, FBLN4
Target/Specificity This EFEMP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 386-414 amino acids from the C-terminal region of human EFEMP2.
Dilution WB~~1:1000
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsEFEMP2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name EFEMP2 (HGNC:3219)
Synonyms FBLN4
Function Plays a crucial role in elastic fiber formation in tissue, and in the formation of ultrastructural connections between elastic laminae and smooth muscle cells in the aorta, therefore participates in terminal differentiation and maturation of smooth muscle cell (SMC) and in the mechanical properties and wall integrity maintenance of the aorta (PubMed:27339457). In addition, is involved in the control of collagen fibril assembly in tissue throught proteolytic activation of LOX leading to cross- linking of collagen and elastin (By similarity). Also promotes ELN coacervation and participates in the deposition of ELN coacervates on to microfibrils but also regulates ELN cross- linking through LOX interaction (PubMed:18973305, PubMed:19570982). Moreover adheres to the cells through heparin binding in a calcium-dependent manner and regulates vascularlar smooth muscle cells proliferation through angiotensin signaling (PubMed:23782690).
Cellular Location Secreted, extracellular space, extracellular matrix Secreted, extracellular space, extracellular matrix, basement membrane {ECO:0000250|UniProtKB:Q9WVJ9}. Note=Localizes on the microfibrils surrounding ELN cores. {ECO:0000250|UniProtKB:Q9WVJ9}
Research Areas
Citations (0)
citation

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Background

EFEMP2 have been found to contain variations of the epidermal growth factor (EGF) domain and have been implicated in functions as diverse as blood coagulation, activation of complement and determination of cell fate during development. The protein contains four EGF2 domains and six calcium-binding EGF2 domains. This protein is necessary for elastic fiber formation and connective tissue development.

References

Chen,Q., et.al., Biochem. J. 423 (1), 79-89 (2009)
Choudhury,R., et.al., J. Biol. Chem. 284 (36), 24553-24567 (2009)
El-Hallous,E., et.al., J. Biol. Chem. 282 (12), 8935-8946 (2007)

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$ 182.50
$ 70.00
Cat# AP9269b
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