GFPT2 Antibody (Center)

Western blot analysis of GFPT2 Antibody (Center) (Cat. #AP9282c) in A2058, K562 cell line lysates (35ug/lane). GFPT2 (arrow) was detected using the purified Pab.
GFPT2 Antibody (Center) (Cat. #AP9282c) IHC analysis in formalin fixed and paraffin embedded human skin followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GFPT2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
GFPT2 Antibody (Center) (Cat. #AP9282c) flow cytometric analysis of A2058 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

SPECIFICATION
CITATIONS
PROTOCOLS
BACKGROUND

Product Information
Application Applications Legend: WB=Western Blot IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin-embedded Sections) IHC-F=Immunohistochemistry (Frozen Sections) IF=Immunofluorescence FC=Flow Cytopmetry IC=Immunochemistry ICC=Immunocytochemistry E=ELISA IP=Immunoprecipitation DB=Dot Blot CHIP=Chromatin Immunoprecipitation FA=Fluorescence Assay IEM=Immunoelectronmicroscopy EIA=Enzyme Immunoassay	FC, IHC-P, WB, E
Primary Accession	O94808
Other Accession	Q4KMC4, Q9Z2Z9, Q08DQ2
Reactivity	Human
Predicted	Bovine, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit Ig
Antigen Region	175-201 aa

Additional Information
Other Names	Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 2, D-fructose-6-phosphate amidotransferase 2, Glutamine:fructose-6-phosphate amidotransferase 2, GFAT 2, GFAT2, Hexosephosphate aminotransferase 2, GFPT2
Target/Specificity	This GFPT2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 175-201 amino acids from the Central region of human GFPT2.
Dilution	WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	GFPT2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	GFPT2
Function	Controls the flux of glucose into the hexosamine pathway. Most likely involved in regulating the availability of precursors for N- and O-linked glycosylation of proteins.
Tissue Location	Highest levels of expression in heart, placenta, and spinal cord EMBL; AB016789; BAA74731.1; -; mRNA EMBL; BT009818; AAP88820.1; -; mRNA EMBL; BC000012; AAH00012.1; -; mRNA CCDS; CCDS43411.1; - RefSeq; NP_005101.1; NM_005110.3 SMR; O94808; - BioGRID; 115270; 37 IntAct; O94808; 11 STRING; 9606.ENSP00000253778; - DrugBank; DB00130; L-Glutamine MEROPS; C44.972; - iPTMnet; O94808; - PhosphoSitePlus; O94808; - BioMuta; GFPT2; - EPD; O94808; - jPOST; O94808; - MassIVE; O94808; - MaxQB; O94808; - PaxDb; O94808; - PeptideAtlas; O94808; - PRIDE; O94808; - ProteomicsDB; 50448; - Antibodypedia; 29588; 190 antibodies DNASU; 9945; - Ensembl; ENST00000253778; ENSP00000253778; ENSG00000131459 GeneID; 9945; - KEGG; hsa:9945; - UCSC; uc003mlw.2; human CTD; 9945; - DisGeNET; 9945; - EuPathDB; HostDB:ENSG00000131459.12; - GeneCards; GFPT2; - HGNC; HGNC:4242; GFPT2 HPA; ENSG00000131459; Tissue enhanced (adipose) MIM; 603865; gene neXtProt; NX_O94808; - OpenTargets; ENSG00000131459; - PharmGKB; PA28652; - eggNOG; KOG1268; Eukaryota GeneTree; ENSGT00940000156413; - HOGENOM; CLU_012520_5_2_1; - InParanoid; O94808; - KO; K00820; - OMA; TSYYSGC; - OrthoDB; 262871at2759; - PhylomeDB; O94808; - TreeFam; TF300864; - PathwayCommons; O94808; - Reactome; R-HSA-446210; Synthesis of UDP-N-acetyl-glucosamine UniPathway; UPA00113; UER00528 BioGRID-ORCS; 9945; 2 hits in 867 CRISPR screens ChiTaRS; GFPT2; human GenomeRNAi; 9945; - Pharos; O94808; Tbio PRO; PR:O94808; - Proteomes; UP000005640; Chromosome 5 RNAct; O94808; protein Bgee; ENSG00000131459; Expressed in layer of synovial tissue and 206 other tissues ExpressionAtlas; O94808; baseline and differential Genevisible; O94808; HS GO; GO:0005829; C:cytosol; TAS:Reactome GO; GO:0097367; F:carbohydrate derivative binding; IEA:InterPro GO; GO:0004360; F:glutamine-fructose-6-phosphate transaminase (isomerizing) activity; IBA:GO_Central GO; GO:1990830; P:cellular response to leukemia inhibitory factor; IEA:Ensembl GO; GO:0006112; P:energy reserve metabolic process; TAS:ProtInc GO; GO:0006002; P:fructose 6-phosphate metabolic process; IBA:GO_Central GO; GO:0006541; P:glutamine metabolic process; IEA:UniProtKB-KW GO; GO:0006487; P:protein N-linked glycosylation; IBA:GO_Central GO; GO:0006048; P:UDP-N-acetylglucosamine biosynthetic process; TAS:Reactome GO; GO:0006047; P:UDP-N-acetylglucosamine metabolic process; IBA:GO_Central CDD; cd05008; SIS_GlmS_GlmD_1; 1 CDD; cd05009; SIS_GlmS_GlmD_2; 1 InterPro; IPR017932; GATase_2_dom InterPro; IPR035466; GlmS/AgaS_SIS InterPro; IPR035490; GlmS/FrlB_SIS InterPro; IPR005855; GlmS_trans InterPro; IPR029055; Ntn_hydrolases_N InterPro; IPR001347; SIS Pfam; PF01380; SIS; 2 SUPFAM; SSF56235; SSF56235; 1 TIGRFAMs; TIGR01135; glmS; 1 PROSITE; PS51278; GATASE_TYPE_2; 1 PROSITE; PS51464; SIS; 2 1: Evidence at protein level; Aminotransferase; Glutamine amidotransferase; Phosphoprotein; Polymorphism; Reference proteome; Repeat; Transferase INIT_MET 1 /note="Removed" /evidence="ECO:0000250" CHAIN 2..682 /note="Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 2" /id="PRO_0000135283" DOMAIN 2..288 /note="Glutamine amidotransferase type-2" /evidence="ECO:0000255\|PROSITE-ProRule:PRU00609" DOMAIN 360..499 /note="SIS 1" /evidence="ECO:0000255\|PROSITE-ProRule:PRU00797" DOMAIN 531..672 /note="SIS 2" /evidence="ECO:0000255\|PROSITE-ProRule:PRU00797" REGION 377..378 /note="Substrate-binding" /evidence="ECO:0000250\|UniProtKB:Q06210" REGION 422..424 /note="Substrate-binding" /evidence="ECO:0000250\|UniProtKB:Q06210" ACT_SITE 2 /note="For GATase activity" /evidence="ECO:0000250\|UniProtKB:P14742" BINDING 427 /note="Substrate" /evidence="ECO:0000250\|UniProtKB:Q06210" BINDING 578 /note="Substrate" /evidence="ECO:0000250\|UniProtKB:Q06210" MOD_RES 244 /note="Phosphoserine" /evidence="ECO:0000244\|PubMed:23186163" VARIANT 471 /note="I -> V (in dbSNP:rs2303007)" /evidence="ECO:0000269\|Ref.2" /id="VAR_013311" SEQUENCE 682 AA; 76931 MW; A090D75526F3192B CRC64; MCGIFAYMNY RVPRTRKEIF ETLIKGLQRL EYRGYDSAGV AIDGNNHEVK ERHIQLVKKR GKVKALDEEL YKQDSMDLKV EFETHFGIAH TRWATHGVPS AVNSHPQRSD KGNEFVVIHN GIITNYKDLR KFLESKGYEF ESETDTETIA KLIKYVFDNR ETEDITFSTL VERVIQQLEG AFALVFKSVH YPGEAVATRR GSPLLIGVRS KYKLSTEQIP ILYRTCTLEN VKNICKTRMK RLDSSACLHA VGDKAVEFFF ASDASAIIEH TNRVIFLEDD DIAAVADGKL SIHRVKRSAS DDPSRAIQTL QMELQQIMKG NFSAFMQKEI FEQPESVFNT MRGRVNFETN TVLLGGLKDH LKEIRRCRRL IVIGCGTSYH AAVATRQVLE ELTELPVMVE LASDFLDRNT PVFRDDVCFF ISQSGETADT LLALRYCKDR GALTVGVTNT VGSSISRETD CGVHINAGPE IGVASTKAYT SQFISLVMFG LMMSEDRISL QNRRQEIIRG LRSLPELIKE VLSLEEKIHD LALELYTQRS LLVMGRGYNY ATCLEGALKI KEITYMHSEG ILAGELKHGP LALIDKQMPV IMVIMKDPCF AKCQNALQQV TARQGRPIIL CSKDDTESSK FAYKTIELPH TVDCLQGILS VIPLQLLSFH LAVLRGYDVD FPRNLAKSVT VE

Research Areas

Citations (0)

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Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

Background

GFPT2 controls the flux of glucose into the hexosamine pathway. This protein most likely involved in regulating the availability of precursors for N-and O-linked glycosylation of proteins.

References

Edwards,T.L., et.al., Ann. Hum. Genet. 74 (2), 97-109 (2010)
Prasad,P., et.al., BMC Med. Genet. 11, 52 (2010)
Srinivasan,V., et.al., Clin. Biochem. 40 (13-14), 952-957 (2007)

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Affinity Purified Rabbit Polyclonal Antibody (Pab)

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References

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