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HCCA2 Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - HCCA2 Antibody (N-term) AP9350a
    Western blot analysis of lysate from HeLa cell line, using HCCA2 Antibody (N-term)(Cat. #AP9350a). AP9350a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.
    detail
  • IHC-P - HCCA2 Antibody (N-term) AP9350a
    Formalin-fixed and paraffin-embedded human testis tissue reacted with HCCA2 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
  • FC - HCCA2 Antibody (N-term) AP9350a
    HCCA2 Antibody (N-term) (Cat. #AP9350a) flow cytometry analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, FC, IHC-P, E
Primary Accession Q70IA6
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 26927 Da
Antigen Region 5-32 aa
Additional Information
Gene ID 81532
Other Names MOB kinase activator 2, HCCA2, Mob2 homolog, Mps one binder kinase activator-like 2, MOB2, HCCA2
Target/Specificity This HCCA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 5-32 amino acids from the N-terminal region of human HCCA2.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHCCA2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MOB2
Synonyms HCCA2
Function Stimulates the autophosphorylation and kinase activity of STK38 and STK38L.
Cellular Location Nucleus. Cytoplasm, perinuclear region
Research Areas
Citations (0)
citation

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Background

HCCA2 binds to and stimulates the kinase activity of the related human serine-threonine kinases NDR1 and NDR2.

References

Devroe,E. J. Biol. Chem. 279 (23), 24444-24451 (2004)
Doyu,M. Brain Res. Mol. Brain Res. 87 (1), 1-11 (2001)
Venter,J.C. Science 291 (5507), 1304-1351 (2001)

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$ 365.00
$ 140.00
Cat# AP9350a
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