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Lgi4 (14M5) Rat Monoclonal Antibody

Lgi4 (14M5) Rat Monoclonal Antibody

     
  •  - Lgi4 (14M5) Rat Monoclonal Antibody AP93629
    IHC-P analysis of mouse cerebellum tissue by anti-mouse Lgi4 antibody (AP93629). IHC-P was performed using sections of the formalin-fixed paraffin-embedded mouse cerebellum tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. The sections were then incubated with anti-mouse Lgi4 primary antibody (AP93629) at 5 µg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG (which cross reacts with rat IgG ) was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control. Result: Purkinje cells are positively stained at cytoplasm of cell body and dendrite.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
IHC
Primary Accession Q8K1S1
Reactivity Mouse
Clonality Monoclonal
Calculated MW 59377 Da
Additional Information
Gene ID 243914
Other Names Leucine-rich repeat LGI family member 4, LGI1-like protein 3, Leucine-rich glioma-inactivated protein 4, Lgi4, Lgil3
Dilution IHC~~1:100~500
Storage Conditions-20℃
Protein Information
Name Lgi4
Synonyms Lgil3
Function Component of Schwann cell signaling pathway(s) that controls axon segregation and myelin formation.
Cellular Location Secreted.
Tissue Location Brain. Expressed in the entire developing peripheral nerves. Strongly expressed in the trigeminal nerve and ganglion and particularly abundant in the boundary cap cells - a transient population of cells that contributes to the Schwann cell population of the dorsal root nerve.
Research Areas
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$ 385.00
Cat# AP93629
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