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Rab6b (14D18) Rat Monoclonal Antibody

Rab6b (14D18) Rat Monoclonal Antibody

     
  •  - Rab6b (14D18) Rat Monoclonal Antibody AP93632
    Various protein samples were run on 6-18% SDS-PAGE under reducing conditions and blotted onto nitrocellulose membrane. AP93632 at 1 µg/mL was used as the primary antibody and peroxidase conjugated goat anti-rat IgG was used as the secondary antibody. Rab6b band was visualized using ECL Substrate. Lane 1: 15 µg of SH-SY5Y lysate Lane 2: 15 µg of mouse brain tissue lysate Lane 3: 15 µg of rat brain tissue lysate Result: AP93632 can detect Rab6b by Western blotting.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession P61294
Reactivity Rat, Mouse
Clonality Monoclonal
Calculated MW 23462 Da
Additional Information
Gene ID 270192
Other Names Ras-related protein Rab-6B, 3.6.5.2, Rab6b, D9Bwg0185e
Dilution WB~~1:1000
Storage Conditions-20℃
Protein Information
Name Rab6b {ECO:0000312|MGI:MGI:107283}
Synonyms D9Bwg0185e
Function The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between active GTP-bound and inactive GDP-bound states. In their active state, drive transport of vesicular carriers from donor organelles to acceptor organelles to regulate the membrane traffic that maintains organelle identity and morphology. Recruits VPS13B to the Golgi membrane. Regulates the compacted morphology of the Golgi. Seems to have a role in retrograde membrane traffic at the level of the Golgi complex. May function in retrograde transport in neuronal cells. Plays a role in neuron projection development.
Cellular Location Golgi apparatus membrane {ECO:0000250|UniProtKB:Q9NRW1}; Lipid-anchor. Endoplasmic reticulum-Golgi intermediate compartment {ECO:0000250|UniProtKB:Q9NRW1}. Cytoplasmic vesicle {ECO:0000250|UniProtKB:Q9NRW1}. Note=Colocalizes with BICD1 at vesicular structures that align along microtubules {ECO:0000250|UniProtKB:Q9NRW1}
Research Areas
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$ 385.00
Cat# AP93632
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