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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   FA96B Antibody (N-term)   

FA96B Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - FA96B Antibody (N-term) AP9370a
    Western blot analysis of FA96B Antibody (N-term) (Cat. #AP9370a) in A375 cell line lysates (35ug/lane). FA96B (arrow) was detected using the purified Pab.
  • IHC-P - FA96B Antibody (N-term) AP9370a
    Formalin-fixed and paraffin-embedded human skin reacted with FA96B Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - FA96B Antibody (N-term) AP9370a
    FA96B Antibody (N-term) (Cat. #AP9370a) flow cytometric analysis of A375 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9Y3D0
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 17663 Da
Antigen Region 16-45 aa
Additional Information
Gene ID 51647
Other Names Mitotic spindle-associated MMXD complex subunit MIP18, MSS19-interacting protein of 18 kDa, Protein FAM96B, FAM96B, MIP18
Target/Specificity This FA96B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 16-45 amino acids from the N-terminal region of human FA96B.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsFA96B Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CIAO2B (HGNC:24261)
Function Component of the cytosolic iron-sulfur protein assembly (CIA) complex, a multiprotein complex that mediates the incorporation of iron-sulfur cluster into extramitochondrial Fe/S proteins (PubMed:23891004, PubMed:22678362, PubMed:22678361, PubMed:29848660). As a CIA complex component and in collaboration with CIAO1 and MMS19, binds to and facilitates the assembly of most cytosolic-nuclear Fe/S proteins (PubMed:23891004, PubMed:29848660). As part of the mitotic spindle-associated MMXD complex it plays a role in chromosome segregation, probably by facilitating iron-sulfur cluster assembly into ERCC2/XPD (PubMed:20797633). Together with MMS19, facilitates the transfer of Fe-S clusters to the motor protein KIF4A, which ensures proper localization of KIF4A to mitotic machinery components to promote the progression of mitosis (PubMed:29848660).
Cellular Location Nucleus. Cytoplasm, cytoskeleton, spindle. Midbody Note=In mitosis, localizes to the spindle during metaphase and the spindle midbody during telophase (PubMed:29848660). Co-localizes with KIF4A to the spindle midzone and midbody during telophase and cytokinesis (PubMed:29848660).
Research Areas
Citations (0)

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Ewing,R.M. Mol. Syst. Biol. 3, 89 (2007)
Wistow,G. Mol. Vis. 8, 171-184 (2002)

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$ 325.00
$ 109.00
Cat# AP9370a
Availability: In Stock
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